Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment
ABSTRACT Lippia origanoides is a honey shrub which has showed hypotensive potential assessed by in vivo studies. The aim of this work is the development of a pharmaceutical formulation composed by an optimized extract obtained from aerial parts of L. origanoides. The quantification of the naringenin...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2018-07-01
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200619&lng=en&tlng=en |
| _version_ | 1818749429973254144 |
|---|---|
| author | Angélica Gomes Coelho José de Sousa Lima Neto Arkellau Kenned Silva Moura Ilmara Cecilia Pinheiro da Silva Morais Francisco Valmor Macedo Cunha Bernardo Melo-Neto Maria das Graças Freire de Medeiros Eilika Andreia Feitosa Vasconcelos Daniel Dias Rufino Arcanjo Lívio Cesar Cunha Nunes Antônia Maria das Graças Lopes Citó |
| author_facet | Angélica Gomes Coelho José de Sousa Lima Neto Arkellau Kenned Silva Moura Ilmara Cecilia Pinheiro da Silva Morais Francisco Valmor Macedo Cunha Bernardo Melo-Neto Maria das Graças Freire de Medeiros Eilika Andreia Feitosa Vasconcelos Daniel Dias Rufino Arcanjo Lívio Cesar Cunha Nunes Antônia Maria das Graças Lopes Citó |
| author_sort | Angélica Gomes Coelho |
| collection | DOAJ |
| description | ABSTRACT Lippia origanoides is a honey shrub which has showed hypotensive potential assessed by in vivo studies. The aim of this work is the development of a pharmaceutical formulation composed by an optimized extract obtained from aerial parts of L. origanoides. The quantification of the naringenin marker in the dry extract and tablets developed was performed, as well as the assessment of the oral acute toxicity in rats. The hydroalcoholic extract of L. origanoides was spray-dried with the addition of colloidal silicon dioxide (Lo-HAE/CSD), and then applied in the preparation of eight different lots of tablets. The influence of the diluent (cellulose or babassu mesocarp), the presence of binder, and the percentage of lubricant, as well as organoleptic and physicochemical characteristics were screened. For the quantification of the marker content both in Lo-HAE/CSD and in the tablets, an analytical curve of the naringenin standard was fitted, and the samples were then analyzed in UFLC. The toxicological assessment was performed in female Wistar rats according to the Acute Toxic Class Method from OECD. The developed tablets produced meet acceptable macroscopic characteristics, and the presence of babassu as diluent provided improved physicochemical properties. The best content of Lo-HAE/CSD in the tablet (100.27%) was identified for the lot containing babassu, composed by 1.0% magnesium stearate, without PVP binder in its formulation. Moreover, Lo-HAE/CSD showed no signs of toxicity. Therefore, the babassu mesocarp powder is a promising pharmaceutical excipient for the development of herbal tablets containing the Lippia origanoides extract. |
| first_indexed | 2024-12-18T04:03:39Z |
| format | Article |
| id | doaj.art-fac2ac5100d44ab9a5989596408df4a6 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-18T04:03:39Z |
| publishDate | 2018-07-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-fac2ac5100d44ab9a5989596408df4a62022-12-21T21:21:38ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902018-07-0154210.1590/s2175-97902018000217226S1984-82502018000200619Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessmentAngélica Gomes CoelhoJosé de Sousa Lima NetoArkellau Kenned Silva MouraIlmara Cecilia Pinheiro da Silva MoraisFrancisco Valmor Macedo CunhaBernardo Melo-NetoMaria das Graças Freire de MedeirosEilika Andreia Feitosa VasconcelosDaniel Dias Rufino ArcanjoLívio Cesar Cunha NunesAntônia Maria das Graças Lopes CitóABSTRACT Lippia origanoides is a honey shrub which has showed hypotensive potential assessed by in vivo studies. The aim of this work is the development of a pharmaceutical formulation composed by an optimized extract obtained from aerial parts of L. origanoides. The quantification of the naringenin marker in the dry extract and tablets developed was performed, as well as the assessment of the oral acute toxicity in rats. The hydroalcoholic extract of L. origanoides was spray-dried with the addition of colloidal silicon dioxide (Lo-HAE/CSD), and then applied in the preparation of eight different lots of tablets. The influence of the diluent (cellulose or babassu mesocarp), the presence of binder, and the percentage of lubricant, as well as organoleptic and physicochemical characteristics were screened. For the quantification of the marker content both in Lo-HAE/CSD and in the tablets, an analytical curve of the naringenin standard was fitted, and the samples were then analyzed in UFLC. The toxicological assessment was performed in female Wistar rats according to the Acute Toxic Class Method from OECD. The developed tablets produced meet acceptable macroscopic characteristics, and the presence of babassu as diluent provided improved physicochemical properties. The best content of Lo-HAE/CSD in the tablet (100.27%) was identified for the lot containing babassu, composed by 1.0% magnesium stearate, without PVP binder in its formulation. Moreover, Lo-HAE/CSD showed no signs of toxicity. Therefore, the babassu mesocarp powder is a promising pharmaceutical excipient for the development of herbal tablets containing the Lippia origanoides extract.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200619&lng=en&tlng=enLippia origanoides/pharmaceutical formulation/toxicity/Babassu mesocarpFactorial planningTablets. |
| spellingShingle | Angélica Gomes Coelho José de Sousa Lima Neto Arkellau Kenned Silva Moura Ilmara Cecilia Pinheiro da Silva Morais Francisco Valmor Macedo Cunha Bernardo Melo-Neto Maria das Graças Freire de Medeiros Eilika Andreia Feitosa Vasconcelos Daniel Dias Rufino Arcanjo Lívio Cesar Cunha Nunes Antônia Maria das Graças Lopes Citó Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment Brazilian Journal of Pharmaceutical Sciences Lippia origanoides/pharmaceutical formulation/toxicity/ Babassu mesocarp Factorial planning Tablets. |
| title | Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment |
| title_full | Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment |
| title_fullStr | Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment |
| title_full_unstemmed | Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment |
| title_short | Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment |
| title_sort | pharmaceutical development of tablets containing a spray dried optimized extract from lippia origanoides h b k influence of excipients and toxicological assessment |
| topic | Lippia origanoides/pharmaceutical formulation/toxicity/ Babassu mesocarp Factorial planning Tablets. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200619&lng=en&tlng=en |
| work_keys_str_mv | AT angelicagomescoelho pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT josedesousalimaneto pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT arkellaukennedsilvamoura pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT ilmaraceciliapinheirodasilvamorais pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT franciscovalmormacedocunha pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT bernardomeloneto pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT mariadasgracasfreiredemedeiros pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT eilikaandreiafeitosavasconcelos pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT danieldiasrufinoarcanjo pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT liviocesarcunhanunes pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment AT antoniamariadasgracaslopescito pharmaceuticaldevelopmentoftabletscontainingaspraydriedoptimizedextractfromlippiaoriganoideshbkinfluenceofexcipientsandtoxicologicalassessment |