Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia
To discover novel antioxidants and anticoagulants for the prevention and treatment of cardiovascular diseases remains ongoing concern. Sagittaria trifolia is a commonly consumed aquatic vegetable with highly nutritious value and wide planting area in China. Polysaccharides are the major bioactive co...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223002745 |
| _version_ | 1797837136592896000 |
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| author | Yang Zhang Yihui Liu Gaoyang Ni Jiahao Xu Yuping Tian Xingyu Liu Jia Gao Qi Gao Yingchao Shen Zhaowei Yan |
| author_facet | Yang Zhang Yihui Liu Gaoyang Ni Jiahao Xu Yuping Tian Xingyu Liu Jia Gao Qi Gao Yingchao Shen Zhaowei Yan |
| author_sort | Yang Zhang |
| collection | DOAJ |
| description | To discover novel antioxidants and anticoagulants for the prevention and treatment of cardiovascular diseases remains ongoing concern. Sagittaria trifolia is a commonly consumed aquatic vegetable with highly nutritious value and wide planting area in China. Polysaccharides are the major bioactive constituents, but little information is available on anticoagulant potential. In this work, a polysaccharide from S. trifolia (PST) was modified by sulfation to produce four sulfated derivatives (SPST-1 ∼ 4), and their antioxidant and anticoagulant potentials were evaluated. Various means of analysis confirmed the success of sulfation on PST. The unmodified PST possessed notable antioxidant and pro-coagulant activities. To some extent, sulfated modification reduced the antioxidant capacity of PST with the increase of degree of substitution (DS), but for the derivative SPST-4 with moderate DS and smallest molecular weight (MW) exerted better antioxidant activity, in particular of scavenging nitrogen-containing radicals. Interestingly, sulfation reversed the innate activity of PST from pro-coagulation to anticoagulation, which highly depended on DS. The derivative SPST-3 with highest DS and moderate MW elicited promising anticoagulant potential, particularly at concentration of 5.00 mg/mL, which was superior to that of heparin (positive control) in the aspect of prolonging thrombin time and prothrombin time. Herein, the present contribution would give evidence that PST and sulfated derivatives SPST-3 and SPST-4 own potentials to be developed as hemostatic, anticoagulant, and antioxidant candidates, respectively. |
| first_indexed | 2024-04-09T15:21:00Z |
| format | Article |
| id | doaj.art-fac41fbb6f164ba6b1dfe735c12e555c |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-09T15:21:00Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-fac41fbb6f164ba6b1dfe735c12e555c2023-04-29T14:49:03ZengElsevierArabian Journal of Chemistry1878-53522023-07-01167104812Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifoliaYang Zhang0Yihui Liu1Gaoyang Ni2Jiahao Xu3Yuping Tian4Xingyu Liu5Jia Gao6Qi Gao7Yingchao Shen8Zhaowei Yan9School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaDepartment of Orthopedics and Traumatology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaDepartment of Orthopedics and Traumatology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, Jiangsu, China; Corresponding authors.Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China; Corresponding authors.To discover novel antioxidants and anticoagulants for the prevention and treatment of cardiovascular diseases remains ongoing concern. Sagittaria trifolia is a commonly consumed aquatic vegetable with highly nutritious value and wide planting area in China. Polysaccharides are the major bioactive constituents, but little information is available on anticoagulant potential. In this work, a polysaccharide from S. trifolia (PST) was modified by sulfation to produce four sulfated derivatives (SPST-1 ∼ 4), and their antioxidant and anticoagulant potentials were evaluated. Various means of analysis confirmed the success of sulfation on PST. The unmodified PST possessed notable antioxidant and pro-coagulant activities. To some extent, sulfated modification reduced the antioxidant capacity of PST with the increase of degree of substitution (DS), but for the derivative SPST-4 with moderate DS and smallest molecular weight (MW) exerted better antioxidant activity, in particular of scavenging nitrogen-containing radicals. Interestingly, sulfation reversed the innate activity of PST from pro-coagulation to anticoagulation, which highly depended on DS. The derivative SPST-3 with highest DS and moderate MW elicited promising anticoagulant potential, particularly at concentration of 5.00 mg/mL, which was superior to that of heparin (positive control) in the aspect of prolonging thrombin time and prothrombin time. Herein, the present contribution would give evidence that PST and sulfated derivatives SPST-3 and SPST-4 own potentials to be developed as hemostatic, anticoagulant, and antioxidant candidates, respectively.http://www.sciencedirect.com/science/article/pii/S1878535223002745PolysaccharideSagittaria trifoliaSulfated modificationAntioxidationAnticoagulationDegree of substitution |
| spellingShingle | Yang Zhang Yihui Liu Gaoyang Ni Jiahao Xu Yuping Tian Xingyu Liu Jia Gao Qi Gao Yingchao Shen Zhaowei Yan Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia Arabian Journal of Chemistry Polysaccharide Sagittaria trifolia Sulfated modification Antioxidation Anticoagulation Degree of substitution |
| title | Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia |
| title_full | Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia |
| title_fullStr | Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia |
| title_full_unstemmed | Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia |
| title_short | Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia |
| title_sort | sulfated modification basic characterization antioxidant and anticoagulant potentials of polysaccharide from sagittaria trifolia |
| topic | Polysaccharide Sagittaria trifolia Sulfated modification Antioxidation Anticoagulation Degree of substitution |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223002745 |
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