Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNA

Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNA

There is an increasing demand for optimization-free multiplex assays to rapidly establish comprehensive target panels for cancer monitoring by liquid biopsy. We present the mediator probe (MP) PCR for the quantification of the seven most frequent point mutations and corresponding wild types (<i&g...

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Bibliographic Details
Main Authors: Franziska Schlenker, Elena Kipf, Max Deuter, Inga Höffkes, Michael Lehnert, Roland Zengerle, Felix von Stetten, Florian Scherer, Julius Wehrle, Nikolas von Bubnoff, Peter Juelg, Tobias Hutzenlaub, Nadine Borst
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:Cancers
Subjects:
mediator probe PCR
digital PCR
multiplex PCR
optimization-free
colorectal carcinoma
standardized universal reporter
Online Access:https://www.mdpi.com/2072-6694/13/22/5742

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https://www.mdpi.com/2072-6694/13/22/5742

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