Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells
To date, the development of anticancer drugs has been conducted using two-dimensional (2D) cell culture systems. However, since cancer cells in the body are generated and developed in three-dimensional (3D) microenvironments, the use of 2D anticancer drug screening can make it difficult to accuratel...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2022-03-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
| Subjects: | |
| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2612&vmd=Full |
| _version_ | 1828907719771291648 |
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| author | Young Jae Lee Keun Cheon Kim Jeong Mook Lim Seung Tae Lee |
| author_facet | Young Jae Lee Keun Cheon Kim Jeong Mook Lim Seung Tae Lee |
| author_sort | Young Jae Lee |
| collection | DOAJ |
| description | To date, the development of anticancer drugs has been conducted using two-dimensional (2D) cell culture systems. However, since cancer cells in the body are generated and developed in three-dimensional (3D) microenvironments, the use of 2D anticancer drug screening can make it difficult to accurately evaluate the anticancer effects of drug candidates. Therefore, as a step towards developing a cancer cell-friendly 3D microenvironment based on a combination of vinylsulfone-functionalized polyethylene glycol (PEG-VS) with dicysteine-containing crosslinker peptides with an intervening matrix metalloproteinase (MMP)-specific cleavage site, the types of MMPs secreted from human hepatocarcinoma HepG2 cells, a representative cancer cell, were analyzed transcriptionally and translationally. MMP3 was confirmed to be the most highly expressed protease secreted by HepG2 cells. This knowledge will be important in the design of a crosslinker necessary for the construction of PEG-based hydrogels customized for the 3D culture of HepG2 cells. |
| first_indexed | 2024-12-13T17:58:11Z |
| format | Article |
| id | doaj.art-facf9c19bdec484eacd116aea25abda5 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-13T17:58:11Z |
| publishDate | 2022-03-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-facf9c19bdec484eacd116aea25abda52022-12-21T23:36:19ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632022-03-01371626610.12750/JARB.37.1.62Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cellsYoung Jae Lee0https://orcid.org/0000-0001-9747-5288Keun Cheon Kim1https://orcid.org/0000-0002-2718-6875Jeong Mook Lim2https://orcid.org/0000-0002-2112-442XSeung Tae Lee3https://orcid.org/0000-0002-8952-3881Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Applied Animal Science, Kangwon National University, Chuncheon 24341, KoreaTo date, the development of anticancer drugs has been conducted using two-dimensional (2D) cell culture systems. However, since cancer cells in the body are generated and developed in three-dimensional (3D) microenvironments, the use of 2D anticancer drug screening can make it difficult to accurately evaluate the anticancer effects of drug candidates. Therefore, as a step towards developing a cancer cell-friendly 3D microenvironment based on a combination of vinylsulfone-functionalized polyethylene glycol (PEG-VS) with dicysteine-containing crosslinker peptides with an intervening matrix metalloproteinase (MMP)-specific cleavage site, the types of MMPs secreted from human hepatocarcinoma HepG2 cells, a representative cancer cell, were analyzed transcriptionally and translationally. MMP3 was confirmed to be the most highly expressed protease secreted by HepG2 cells. This knowledge will be important in the design of a crosslinker necessary for the construction of PEG-based hydrogels customized for the 3D culture of HepG2 cells.https://www.e-jarb.org/journal/view.html?uid=2612&vmd=Fullhepatocarcinoma cellshydrogelmatrix metalloproteinasethree-dimension culture |
| spellingShingle | Young Jae Lee Keun Cheon Kim Jeong Mook Lim Seung Tae Lee Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells Journal of Animal Reproduction and Biotechnology hepatocarcinoma cells hydrogel matrix metalloproteinase three-dimension culture |
| title | Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells |
| title_full | Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells |
| title_fullStr | Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells |
| title_full_unstemmed | Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells |
| title_short | Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells |
| title_sort | identification of matrix metalloproteinases secreted by human hepatocarcinoma hepg2 cells |
| topic | hepatocarcinoma cells hydrogel matrix metalloproteinase three-dimension culture |
| url | https://www.e-jarb.org/journal/view.html?uid=2612&vmd=Full |
| work_keys_str_mv | AT youngjaelee identificationofmatrixmetalloproteinasessecretedbyhumanhepatocarcinomahepg2cells AT keuncheonkim identificationofmatrixmetalloproteinasessecretedbyhumanhepatocarcinomahepg2cells AT jeongmooklim identificationofmatrixmetalloproteinasessecretedbyhumanhepatocarcinomahepg2cells AT seungtaelee identificationofmatrixmetalloproteinasessecretedbyhumanhepatocarcinomahepg2cells |