A TurboID-based proximity labelling approach for identifying the DNA-binding proteins

Summary: Biotin proximity labeling is a technique based on the TurboID enzyme that can be used to capture weak or dynamic interactions that had previously not been used to map proteins interacting with a specific DNA sequence. Here, we present a protocol for identifying specific DNA-sequence-binding...

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Bibliographic Details
Main Authors: Xia-fei Wei, Shan Li, Jie-li Hu
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000977