Cryopreservation and post-thaw characterization of dissociated human islet cells.

The objective of this study is to optimize the cryopreservation of dissociated islet cells and obtain functional cells that can be used in single-cell transcriptome studies on the pathology and treatment of diabetes. Using an iterative graded freezing approach we obtained viable cells after cooling...

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Bibliographic Details
Main Authors: Leah A Marquez-Curtis, Xiao-Qing Dai, Yan Hang, Jonathan Y Lam, James Lyon, Jocelyn E Manning Fox, Locksley E McGann, Patrick E MacDonald, Seung K Kim, Janet A W Elliott
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0263005