Isolation and RNA sequencing of single nuclei from Drosophila tissues

Isolation and RNA sequencing of single nuclei from Drosophila tissues

Summary: Many insect cells are encapsulated within the exoskeleton and cannot be dissociated intact, making them inaccessible to single-cell transcriptomic profiling. We have used single-nucleus RNA sequencing to extract transcriptomic information from multiple Drosophila tissues. Here, we describe...

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Bibliographic Details
Main Authors: Colleen N. McLaughlin, Yanyan Qi, Stephen R. Quake, Liqun Luo, Hongjie Li
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Genomics
Model Organisms
RNAseq
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002970

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http://www.sciencedirect.com/science/article/pii/S2666166722002970

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