Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox Status
Obesity causes insulin resistance and hyperinsulinemia which causes skeletal muscle dysfunction resulting in a decrease in contraction force and a reduced capacity to avoid fatigue, which overall, causes an increase in oxidative stress. K<sub>ATP</sub> channel openers such as diazoxide a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/12/1232 |
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| author | Mariana Gómez-Barroso Koré M. Moreno-Calderón Elizabeth Sánchez-Duarte Christian Cortés-Rojo Alfredo Saavedra-Molina Alain R. Rodríguez-Orozco Rocío Montoya-Pérez |
| author_facet | Mariana Gómez-Barroso Koré M. Moreno-Calderón Elizabeth Sánchez-Duarte Christian Cortés-Rojo Alfredo Saavedra-Molina Alain R. Rodríguez-Orozco Rocío Montoya-Pérez |
| author_sort | Mariana Gómez-Barroso |
| collection | DOAJ |
| description | Obesity causes insulin resistance and hyperinsulinemia which causes skeletal muscle dysfunction resulting in a decrease in contraction force and a reduced capacity to avoid fatigue, which overall, causes an increase in oxidative stress. K<sub>ATP</sub> channel openers such as diazoxide and the implementation of exercise protocols have been reported to be actively involved in protecting skeletal muscle against metabolic stress; however, the effects of diazoxide and exercise on muscle contraction and oxidative stress during obesity have not been explored. This study aimed to determine the effect of diazoxide in the contraction of skeletal muscle of obese male Wistar rats (35 mg/kg), and with an exercise protocol (five weeks) and the combination from both. Results showed that the treatment with diazoxide and exercise improved muscular contraction, showing an increase in maximum tension and total tension due to decreased ROS and lipid peroxidation levels and improved glutathione redox state. Therefore, these results suggest that diazoxide and exercise improve muscle function during obesity, possibly through its effects as K<sub>ATP</sub> channel openers. |
| first_indexed | 2024-03-10T14:19:01Z |
| format | Article |
| id | doaj.art-fae5356ac8c743c08e6b3cd77aeed8c9 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T14:19:01Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-fae5356ac8c743c08e6b3cd77aeed8c92023-11-20T23:34:39ZengMDPI AGAntioxidants2076-39212020-12-01912123210.3390/antiox9121232Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox StatusMariana Gómez-Barroso0Koré M. Moreno-Calderón1Elizabeth Sánchez-Duarte2Christian Cortés-Rojo3Alfredo Saavedra-Molina4Alain R. Rodríguez-Orozco5Rocío Montoya-Pérez6Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia, Michoacán 58030, MexicoInstituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia, Michoacán 58030, MexicoDepartamento de Ciencias Aplicadas al Trabajo, Universidad de Guanajuato Campus León, Eugenio Garza Sada 572, Lomas del Campestre Sección 2, León, Guanajuato 37150, MexicoInstituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia, Michoacán 58030, MexicoInstituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia, Michoacán 58030, MexicoFacultad de Ciencias Médicas y Biológicas “Dr. Ignacio Chávez”, Universidad Michoacana de San Nicolás de Hidalgo Av. Dr. Rafael Carrillo S/N, Esq. Dr. Salvador González Herrejón, Bosque Cuauhtémoc, Morelia, Michoacán 58020, MexicoInstituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia, Michoacán 58030, MexicoObesity causes insulin resistance and hyperinsulinemia which causes skeletal muscle dysfunction resulting in a decrease in contraction force and a reduced capacity to avoid fatigue, which overall, causes an increase in oxidative stress. K<sub>ATP</sub> channel openers such as diazoxide and the implementation of exercise protocols have been reported to be actively involved in protecting skeletal muscle against metabolic stress; however, the effects of diazoxide and exercise on muscle contraction and oxidative stress during obesity have not been explored. This study aimed to determine the effect of diazoxide in the contraction of skeletal muscle of obese male Wistar rats (35 mg/kg), and with an exercise protocol (five weeks) and the combination from both. Results showed that the treatment with diazoxide and exercise improved muscular contraction, showing an increase in maximum tension and total tension due to decreased ROS and lipid peroxidation levels and improved glutathione redox state. Therefore, these results suggest that diazoxide and exercise improve muscle function during obesity, possibly through its effects as K<sub>ATP</sub> channel openers.https://www.mdpi.com/2076-3921/9/12/1232skeletal muscleobesityfatigueoxidative stressdiazoxideexercise |
| spellingShingle | Mariana Gómez-Barroso Koré M. Moreno-Calderón Elizabeth Sánchez-Duarte Christian Cortés-Rojo Alfredo Saavedra-Molina Alain R. Rodríguez-Orozco Rocío Montoya-Pérez Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox Status Antioxidants skeletal muscle obesity fatigue oxidative stress diazoxide exercise |
| title | Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox Status |
| title_full | Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox Status |
| title_fullStr | Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox Status |
| title_full_unstemmed | Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox Status |
| title_short | Diazoxide and Exercise Enhance Muscle Contraction during Obesity by Decreasing ROS Levels, Lipid Peroxidation, and Improving Glutathione Redox Status |
| title_sort | diazoxide and exercise enhance muscle contraction during obesity by decreasing ros levels lipid peroxidation and improving glutathione redox status |
| topic | skeletal muscle obesity fatigue oxidative stress diazoxide exercise |
| url | https://www.mdpi.com/2076-3921/9/12/1232 |
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