Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

Abstract Background One ongoing concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-target activity is challenging. Here, we present SMRT-OTS and Nano-OTS, two novel, amplification-free,...

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Bibliographic Details
Main Authors: Ida Höijer, Josefin Johansson, Sanna Gudmundsson, Chen-Shan Chin, Ignas Bunikis, Susana Häggqvist, Anastasia Emmanouilidou, Maria Wilbe, Marcel den Hoed, Marie-Louise Bondeson, Lars Feuk, Ulf Gyllensten, Adam Ameur
Format: Article
Language:English
Published: BMC 2020-12-01
Series:Genome Biology
Subjects:
CRISPR-Cas9
On-target
Off-target
Long-read sequencing
Single molecule sequencing
PacBio sequencing
Online Access:https://doi.org/10.1186/s13059-020-02206-w

Internet

https://doi.org/10.1186/s13059-020-02206-w

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