A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19

A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19

Summary: This protocol describes a flow cytometry approach to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in COVID-19-positive patient sera samples without the need of specific laboratory facilities for viral infection. We developed a human-cell-based system using spike-exp...

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Bibliographic Details
Main Authors: Sophie Martin, Gwénaële Jégou, Aurore Nicolas, Matthieu Le Gallo, Éric Chevet, Florence Godey, Tony Avril
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Flow Cytometry/Mass Cytometry
Cell-based Assays
Health Sciences
Microbiology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001095

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http://www.sciencedirect.com/science/article/pii/S2666166722001095

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