Massively targeted evaluation of therapeutic CRISPR off-targets in cells

Massively targeted evaluation of therapeutic CRISPR off-targets in cells

Thorough evaluation of CRISPR RNA-guided nucleases off-targets in cells is required for advancing gene therapies. Here the authors report SURRO-seq for the simultaneous investigation of thousands of off-target sites for therapeutic RNA-guided nucleases in cells.

Bibliographic Details
Main Authors: Xiaoguang Pan, Kunli Qu, Hao Yuan, Xi Xiang, Christian Anthon, Liubov Pashkova, Xue Liang, Peng Han, Giulia I. Corsi, Fengping Xu, Ping Liu, Jiayan Zhong, Yan Zhou, Tao Ma, Hui Jiang, Junnian Liu, Jian Wang, Niels Jessen, Lars Bolund, Huanming Yang, Xun Xu, George M. Church, Jan Gorodkin, Lin Lin, Yonglun Luo
Format: Article
Language:English
Published: Nature Portfolio 2022-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-31543-6

Internet

https://doi.org/10.1038/s41467-022-31543-6

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