Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection

Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection

Samples for histological analyses are often formalin-fixed paraffin-embedded (FFPE) and slide-mounted, which complicates RNA extraction for many downstream molecular applications. Furthermore, when the region of interest is extremely small due to isolation with laser microdissection (LMD), extractin...

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Bibliographic Details
Main Authors: Alexander Paping, Clara Basler, Rebecca C Rancourt, Loreen Ehrlich, Kerstin Melchior, Wolfgang Henrich, Thorsten Braun
Format: Article
Language:English
Published: Taylor & Francis Group 2022-06-01
Series:BioTechniques
Subjects:
FFPE
laser microdissection
LMD
qRT-PCR
RNA expression
RNA isolation
Online Access:https://www.future-science.com/doi/10.2144/btn-2022-0026

Internet

https://www.future-science.com/doi/10.2144/btn-2022-0026

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