Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles

Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles

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ABSTRACT Shotgun metagenomic sequencing has transformed our understanding of microbial community ecology. However, preparing metagenomic libraries for high-throughput DNA sequencing remains a costly, labor-intensive, and time-consuming procedure, which in turn limits the utility of metagenomes. Seve...

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Bibliographic Details
Main Authors: Christopher A. Gaulke, Emily R. Schmeltzer, Mark Dasenko, Brett M. Tyler, Rebecca Vega Thurber, Thomas J. Sharpton
Format: Article
Language:English
Published: American Society for Microbiology 2021-10-01
Series:mSystems
Subjects:
coral
gut
library preparation
metagenomics
microbiome
next-generation sequencing
Online Access:https://journals.asm.org/doi/10.1128/msystems.00440-21

Internet

https://journals.asm.org/doi/10.1128/msystems.00440-21

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