Dynamic and single cell characterization of a CRISPR-interference toolset in Pseudomonas putida KT2440 for β-ketoadipate production from p-coumarate

Dynamic and single cell characterization of a CRISPR-interference toolset in Pseudomonas putida KT2440 for β-ketoadipate production from p-coumarate

Pseudomonas putida KT2440 is a well-studied bacterium for the conversion of lignin-derived aromatic compounds to bioproducts. The development of advanced genetic tools in P. putida has reduced the turnaround time for hypothesis testing and enabled the construction of strains capable of producing var...

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Bibliographic Details
Main Authors: Jacob A. Fenster, Allison Z. Werner, Jian Wei Tay, Matthew Gillen, Leo Schirokauer, Nicholas C. Hill, Audrey Watson, Kelsey J. Ramirez, Christopher W. Johnson, Gregg T. Beckham, Jeffrey C. Cameron, Carrie A. Eckert
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Metabolic Engineering Communications
Subjects:
CRISPR interference
Single-cell analysis
Microbial lignin valorization
Pseudomonas putida KT2440
Heterogeneity
Online Access:http://www.sciencedirect.com/science/article/pii/S221403012200013X

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http://www.sciencedirect.com/science/article/pii/S221403012200013X

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