B-cell lymphopoiesis is regulated by cathepsin L.
Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSL(nkt/nkt) mice that lack CTSL activity, we have previously demonstrated that th...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3621861?pdf=render |
| _version_ | 1811330432273219584 |
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| author | Maria Noel Badano Gabriela Lorena Camicia Gabriela Lombardi Andrea Maglioco Gabriel Cabrera Hector Costa Roberto Pablo Meiss Isabel Piazzon Irene Nepomnaschy |
| author_facet | Maria Noel Badano Gabriela Lorena Camicia Gabriela Lombardi Andrea Maglioco Gabriel Cabrera Hector Costa Roberto Pablo Meiss Isabel Piazzon Irene Nepomnaschy |
| author_sort | Maria Noel Badano |
| collection | DOAJ |
| description | Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSL(nkt/nkt) mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSL (nkt/nkt) mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSL (nkt/nkt) mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, in vitro and in vivo experiments showed that BM B-cell production was markedly increased in CTSL (nkt/nkt) mice. Besides, BM B-cell emigration to the spleen was increased in CTSL (nkt/nkt) mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSL (nkt/nkt) mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells. |
| first_indexed | 2024-04-13T16:02:38Z |
| format | Article |
| id | doaj.art-fb460282bf724fadacab445676e55e67 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T16:02:38Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fb460282bf724fadacab445676e55e672022-12-22T02:40:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6134710.1371/journal.pone.0061347B-cell lymphopoiesis is regulated by cathepsin L.Maria Noel BadanoGabriela Lorena CamiciaGabriela LombardiAndrea MagliocoGabriel CabreraHector CostaRoberto Pablo MeissIsabel PiazzonIrene NepomnaschyCathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSL(nkt/nkt) mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSL (nkt/nkt) mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSL (nkt/nkt) mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, in vitro and in vivo experiments showed that BM B-cell production was markedly increased in CTSL (nkt/nkt) mice. Besides, BM B-cell emigration to the spleen was increased in CTSL (nkt/nkt) mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSL (nkt/nkt) mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.http://europepmc.org/articles/PMC3621861?pdf=render |
| spellingShingle | Maria Noel Badano Gabriela Lorena Camicia Gabriela Lombardi Andrea Maglioco Gabriel Cabrera Hector Costa Roberto Pablo Meiss Isabel Piazzon Irene Nepomnaschy B-cell lymphopoiesis is regulated by cathepsin L. PLoS ONE |
| title | B-cell lymphopoiesis is regulated by cathepsin L. |
| title_full | B-cell lymphopoiesis is regulated by cathepsin L. |
| title_fullStr | B-cell lymphopoiesis is regulated by cathepsin L. |
| title_full_unstemmed | B-cell lymphopoiesis is regulated by cathepsin L. |
| title_short | B-cell lymphopoiesis is regulated by cathepsin L. |
| title_sort | b cell lymphopoiesis is regulated by cathepsin l |
| url | http://europepmc.org/articles/PMC3621861?pdf=render |
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