Solubilization, Activation and Partial Purification of a Sialidase from Horse Liver
Using sialyl-methylumbelliferyl α-glycoside as substrate, sialidase in horse liver was detected as a membrane-bound enzyme. A yield of about 50% of sialidase activity was found in supernatant when solubilized in 0.1 M sodium-phosphate buffer pH 5.5, containing 0.15 M NaCl, 0.25 M sucrose, and 0.5% T...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2005-09-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S197830191630331X |