Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation

Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation

Gene-modified animals, including pigs, can be generated efficiently by introducing CRISPR associated protein 9 (CRISPR/Cas9) into zygotes. However, in many cases, these zygotes tend to become mosaic mutants with various different mutant cell types, making it difficult to analyze the phenotype of gen...

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Bibliographic Details
Main Authors: Shiro YAMASHITA, Yuhei KOGASAKA, Yuuki HIRADATE, Kentaro TANEMURA, Yutaka SENDAI
Format: Article
Language:English
Published: The Society for Reproduction and Development 2019-11-01
Series:The Journal of Reproduction and Development
Subjects:
crispr associated protein 9 (crispr/cas9)
electroporation
gene editing
mosaic mutants
three-prime repair exonuclease 2 (trex2) exonuclease
Online Access:https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-088/_pdf/-char/en

Internet

https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-088/_pdf/-char/en

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