Storage media and RNA extraction approaches substantially influence the recovery and integrity of livestock fecal microbial RNA

Storage media and RNA extraction approaches substantially influence the recovery and integrity of livestock fecal microbial RNA

Background There is growing interest in understanding gut microbiome dynamics, to increase the sustainability of livestock production systems and to better understand the dynamics that regulate antibiotic resistance genes (i.e., the resistome). High-throughput sequencing of RNA transcripts (RNA-seq)...

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Bibliographic Details
Main Authors: Raju Koorakula, Mahdi Ghanbari, Matteo Schiavinato, Gertrude Wegl, Juliane C. Dohm, Konrad J. Domig
Format: Article
Language:English
Published: PeerJ Inc. 2022-06-01
Series:PeerJ
Subjects:
Livestock microbiome
Metatranscriptomics
Sample storage
RNA-extraction
Chicken feces
Pig feces
Online Access:https://peerj.com/articles/13547.pdf

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https://peerj.com/articles/13547.pdf

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