Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

Abstract Background CRISPR/Cas9 mediated genome editing has expedited the way of constructing multiple gene alterations in filamentous fungi, whereas traditional methods are time-consuming and can be of mutagenic nature. These developments allow the study of large gene families that contain putative...

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Bibliographic Details
Main Authors: Tim M. van Leeuwe, Mark Arentshorst, Tim Ernst, Ebru Alazi, Peter J. Punt, Arthur F. J. Ram
Format: Article
Language:English
Published: BMC 2019-09-01
Series:Fungal Biology and Biotechnology
Subjects:
CRISPR/Cas9
Gene editing
Marker free
Multiplexing
Knockouts
Gene families
Online Access:http://link.springer.com/article/10.1186/s40694-019-0076-7

Internet

http://link.springer.com/article/10.1186/s40694-019-0076-7

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