Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

Here, we illustrate what happens inside the catalytic cleft of an enzyme when substrate or ligand binds on single-millisecond timescales. The initial phase of the enzymatic cycle is observed with near-atomic resolution using the most advanced X-ray source currently available: the European XFEL (EuXF...

Full description

Bibliographic Details
Main Authors: Suraj Pandey, George Calvey, Andrea M. Katz, Tek Narsingh Malla, Faisal H. M. Koua, Jose M. Martin-Garcia, Ishwor Poudyal, Jay-How Yang, Mohammad Vakili, Oleksandr Yefanov, Kara A. Zielinski, Sasa Bajt, Salah Awel, Katarina Doerner, Matthias Frank, Luca Gelisio, Rebecca Jernigan, Henry Kirkwood, Marco Kloos, Jayanath Koliyadu, Valerio Mariani, Mitchell D. Miller, Grant Mills, Garrett Nelson, Jose L. Olmos Jr, Alireza Sadri, Tokushi Sato, Alexandra Tolstikova, Weijun Xu, Abbas Ourmazd, John C. H. Spence, Peter Schwander, Anton Barty, Henry N. Chapman, Petra Fromme, Adrian P. Mancuso, George N. Phillips Jr, Richard Bean, Lois Pollack, Marius Schmidt
Format: Article
Language:English
Published: International Union of Crystallography 2021-11-01
Series:IUCrJ
Subjects:
substrate diffusion in crystals
antibiotic resistance
β-lactamases
enzyme kinetics
irreversible inhibition
mix-and-inject serial crystallography
serial femtosecond crystallography
european x-ray free-electron laser
megahertz pulse-repetition rate
protein structure determination
drug discovery
ceftriaxone
sulbactam
x-ray crystallography
enzyme mechanisms
Online Access:http://scripts.iucr.org/cgi-bin/paper?S2052252521008125

Internet

http://scripts.iucr.org/cgi-bin/paper?S2052252521008125

Similar Items