Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult mice
ABSTRACT Objective: We hypothesized that perinatal manipulations of the nitrergic system would affect adult animal behaviors. Methods: We tested this hypothesis by perinatally administering N(G)-Nitro-L-arginine methyl ester (L-NAME), a non-specific antagonist of nitric oxide synthase for 15 days...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2023-12-01
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| Series: | Einstein (São Paulo) |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082023000100243&lng=en&tlng=en |
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| author | Raoni Conceição Dos-Santos Cláudio da Silva-Almeida Bruno Guimarães Marinho Rodrigo Rodrigues da Conceição Wellington da Silva Côrtes Ragab Gaber Ahmed Roberto Laureano-Melo |
| author_facet | Raoni Conceição Dos-Santos Cláudio da Silva-Almeida Bruno Guimarães Marinho Rodrigo Rodrigues da Conceição Wellington da Silva Côrtes Ragab Gaber Ahmed Roberto Laureano-Melo |
| author_sort | Raoni Conceição Dos-Santos |
| collection | DOAJ |
| description | ABSTRACT Objective: We hypothesized that perinatal manipulations of the nitrergic system would affect adult animal behaviors. Methods: We tested this hypothesis by perinatally administering N(G)-Nitro-L-arginine methyl ester (L-NAME), a non-specific antagonist of nitric oxide synthase for 15 days and assessed anxiety- and depression-like behaviors in adult mice. At 70 days of age, the mice were subjected to a battery of tests consisting of the open-field, light/dark box, forced swim, and tail-flick tests. The tests were performed at two-day intervals, and the order of the tests within the battery was determined according to the progressive invasiveness degree. Results: L-NAME-treated animals exhibited decreased anxiety-like behavior in the light/dark box and open field tests, with no change in locomotor activity. Additionally, they demonstrated decreased depression-like behavior in the forced swim test and no change in pain perception in the tail-flick test. Conclusion: The nitrergic system is possibly involved in neural circuitry development that regulates behaviors since blocking perinatal nitric oxide production decreases anxiety- and depression-like behaviors in adult mice. |
| first_indexed | 2024-03-09T02:54:40Z |
| format | Article |
| id | doaj.art-fbef921875b641b9928f1406273bf8be |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-03-09T02:54:40Z |
| publishDate | 2023-12-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-fbef921875b641b9928f1406273bf8be2023-12-05T07:53:26ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-63852023-12-012110.31744/einstein_journal/2023ao0302Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult miceRaoni Conceição Dos-Santoshttps://orcid.org/0000-0001-9675-754XCláudio da Silva-Almeidahttps://orcid.org/0000-0002-4393-997XBruno Guimarães Marinhohttps://orcid.org/0000-0002-0113-1921Rodrigo Rodrigues da Conceiçãohttps://orcid.org/0000-0002-1260-4296Wellington da Silva Côrteshttps://orcid.org/0000-0002-2359-5073Ragab Gaber Ahmedhttps://orcid.org/0000-0001-5644-8291Roberto Laureano-Melohttps://orcid.org/0000-0001-6146-3971ABSTRACT Objective: We hypothesized that perinatal manipulations of the nitrergic system would affect adult animal behaviors. Methods: We tested this hypothesis by perinatally administering N(G)-Nitro-L-arginine methyl ester (L-NAME), a non-specific antagonist of nitric oxide synthase for 15 days and assessed anxiety- and depression-like behaviors in adult mice. At 70 days of age, the mice were subjected to a battery of tests consisting of the open-field, light/dark box, forced swim, and tail-flick tests. The tests were performed at two-day intervals, and the order of the tests within the battery was determined according to the progressive invasiveness degree. Results: L-NAME-treated animals exhibited decreased anxiety-like behavior in the light/dark box and open field tests, with no change in locomotor activity. Additionally, they demonstrated decreased depression-like behavior in the forced swim test and no change in pain perception in the tail-flick test. Conclusion: The nitrergic system is possibly involved in neural circuitry development that regulates behaviors since blocking perinatal nitric oxide production decreases anxiety- and depression-like behaviors in adult mice.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082023000100243&lng=en&tlng=enAnxietyDepressionNG-nitroarginine methyl esterNitric oxidePain perceptionMice |
| spellingShingle | Raoni Conceição Dos-Santos Cláudio da Silva-Almeida Bruno Guimarães Marinho Rodrigo Rodrigues da Conceição Wellington da Silva Côrtes Ragab Gaber Ahmed Roberto Laureano-Melo Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult mice Einstein (São Paulo) Anxiety Depression NG-nitroarginine methyl ester Nitric oxide Pain perception Mice |
| title | Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult mice |
| title_full | Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult mice |
| title_fullStr | Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult mice |
| title_full_unstemmed | Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult mice |
| title_short | Perinatal N(G)-Nitro-L-arginine methyl ester administration decreases anxiety- and depression-like behaviors in adult mice |
| title_sort | perinatal n g nitro l arginine methyl ester administration decreases anxiety and depression like behaviors in adult mice |
| topic | Anxiety Depression NG-nitroarginine methyl ester Nitric oxide Pain perception Mice |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082023000100243&lng=en&tlng=en |
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