PCR-based gene synthesis with overlapping unisense-oligomers asymmetric extension supported by a simulator for oligonucleotide extension achieved 1 kbp dsDNA
We formulated a method to synthesize 1 kbp DNA fragments using ‘oligomer unidirectional joining method’ via asymmetric extension supported by a simulator for oligonucleotide extension (AESOE). In this study, trials were conducted on 41 sets of different genomic pieces of ten flaviviral genomes, and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-06-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0127 |