Dark coffee consumption protects human blood cells from spontaneous DNA damage
Coffee increasingly attracts notice with respect to beneficial health effects. Our objective was to investigate DNA protective effects of a special roast coffee blend of pure Arabica (Coffea arabica L.) in healthy volunteers (n = 96), following a prospective, randomized, controlled study with parall...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464619300702 |
| _version_ | 1818415719877967872 |
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| author | Gudrun Pahlke Eva Attakpah Georg Aichinger Katarina Ahlberg Christina Maria Hochkogler Kerstin Schweiger Dorothea Schipp Veronika Somoza Doris Marko |
| author_facet | Gudrun Pahlke Eva Attakpah Georg Aichinger Katarina Ahlberg Christina Maria Hochkogler Kerstin Schweiger Dorothea Schipp Veronika Somoza Doris Marko |
| author_sort | Gudrun Pahlke |
| collection | DOAJ |
| description | Coffee increasingly attracts notice with respect to beneficial health effects. Our objective was to investigate DNA protective effects of a special roast coffee blend of pure Arabica (Coffea arabica L.) in healthy volunteers (n = 96), following a prospective, randomized, controlled study with parallel design (coffee versus water). Potential modulation of Nrf2 signaling was evaluated by focusing on its two master regulators, Nrf2 and Keap1, as well as on Nrf2 translocation in the volunteers’ lymphocytes (PBLs). In this context a newly established fluorescence imaging method for Nrf2 translocation analysis in PBLs turned out as feasible and eligible tool applicable for future studies.After chronical coffee consumption (8 weeks) spontaneous DNA strand breaks were significantly lower in the coffee group compared to water control, suggesting a protective effect of the coffee blend. Nrf2 signaling was remotely affected, indicating that additional mechanisms of protection from DNA damage need to be considered. |
| first_indexed | 2024-12-14T11:39:28Z |
| format | Article |
| id | doaj.art-fc002da83e3a413db7d3a1158c3caee7 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T11:39:28Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-fc002da83e3a413db7d3a1158c3caee72022-12-21T23:02:54ZengElsevierJournal of Functional Foods1756-46462019-04-0155285295Dark coffee consumption protects human blood cells from spontaneous DNA damageGudrun Pahlke0Eva Attakpah1Georg Aichinger2Katarina Ahlberg3Christina Maria Hochkogler4Kerstin Schweiger5Dorothea Schipp6Veronika Somoza7Doris Marko8Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, AustriaDepartment of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, AustriaDepartment of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, AustriaDepartment of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, AustriaDepartment of Physiological Chemistry, Christian Doppler Laboratory for Bioactive Aroma Compounds, Faculty of Chemistry, University of Vienna, Althanstr. 14/UZA II, A-1090 Vienna, AustriaDepartment of Physiological Chemistry, Christian Doppler Laboratory for Bioactive Aroma Compounds, Faculty of Chemistry, University of Vienna, Althanstr. 14/UZA II, A-1090 Vienna, AustriaDs Statistik, Rosenthal-Bielatal, GermanyDepartment of Physiological Chemistry, Christian Doppler Laboratory for Bioactive Aroma Compounds, Faculty of Chemistry, University of Vienna, Althanstr. 14/UZA II, A-1090 Vienna, AustriaDepartment of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, Austria; Corresponding author.Coffee increasingly attracts notice with respect to beneficial health effects. Our objective was to investigate DNA protective effects of a special roast coffee blend of pure Arabica (Coffea arabica L.) in healthy volunteers (n = 96), following a prospective, randomized, controlled study with parallel design (coffee versus water). Potential modulation of Nrf2 signaling was evaluated by focusing on its two master regulators, Nrf2 and Keap1, as well as on Nrf2 translocation in the volunteers’ lymphocytes (PBLs). In this context a newly established fluorescence imaging method for Nrf2 translocation analysis in PBLs turned out as feasible and eligible tool applicable for future studies.After chronical coffee consumption (8 weeks) spontaneous DNA strand breaks were significantly lower in the coffee group compared to water control, suggesting a protective effect of the coffee blend. Nrf2 signaling was remotely affected, indicating that additional mechanisms of protection from DNA damage need to be considered.http://www.sciencedirect.com/science/article/pii/S1756464619300702CoffeeDNA integrityNrf2Keap1human PBLs |
| spellingShingle | Gudrun Pahlke Eva Attakpah Georg Aichinger Katarina Ahlberg Christina Maria Hochkogler Kerstin Schweiger Dorothea Schipp Veronika Somoza Doris Marko Dark coffee consumption protects human blood cells from spontaneous DNA damage Journal of Functional Foods Coffee DNA integrity Nrf2 Keap1 human PBLs |
| title | Dark coffee consumption protects human blood cells from spontaneous DNA damage |
| title_full | Dark coffee consumption protects human blood cells from spontaneous DNA damage |
| title_fullStr | Dark coffee consumption protects human blood cells from spontaneous DNA damage |
| title_full_unstemmed | Dark coffee consumption protects human blood cells from spontaneous DNA damage |
| title_short | Dark coffee consumption protects human blood cells from spontaneous DNA damage |
| title_sort | dark coffee consumption protects human blood cells from spontaneous dna damage |
| topic | Coffee DNA integrity Nrf2 Keap1 human PBLs |
| url | http://www.sciencedirect.com/science/article/pii/S1756464619300702 |
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