Validation of Promoters and Codon Optimization on CRISPR/Cas9-Engineered Jurkat Cells Stably Expressing αRep4E3 for Interfering with HIV-1 Replication

Validation of Promoters and Codon Optimization on CRISPR/Cas9-Engineered Jurkat Cells Stably Expressing αRep4E3 for Interfering with HIV-1 Replication

Persistent and efficient therapeutic protein expression in the specific target cell is a significant concern in gene therapy. The controllable integration site, suitable promoter, and proper codon usage influence the effectiveness of the therapeutic outcome. Previously, we developed a non-immunoglob...

Full description

Bibliographic Details
Main Authors: Koollawat Chupradit, Kanokporn Sornsuwan, Kritayaporn Saiprayong, Methichit Wattanapanitch, Chatchai Tayapiwatana
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
alpha repeat protein
CRISPR/Cas9
promoter
codon optimization
HIV-1
Online Access:https://www.mdpi.com/1422-0067/23/23/15049

Internet

https://www.mdpi.com/1422-0067/23/23/15049

Similar Items