Transformation of Penicillium rubens 212 and Expression of GFP and DsRED Coding Genes for Visualization of Plant-Biocontrol Agent Interaction

Transformation of Penicillium rubens 212 and Expression of GFP and DsRED Coding Genes for Visualization of Plant-Biocontrol Agent Interaction

Strain 212 of Penicillium rubens (PO212) is an effective fungal biological control agent against a broad spectrum of diseases of horticultural plants. A pyrimidine auxotrophic isolate of PO212, PO212_18.2, carrying an inactive pyrG gene, has been used as host for transformation by positive selection...

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Bibliographic Details
Main Authors: Maria Villarino, Eduardo A. Espeso, Paloma Melgarejo, Inmaculada Larena
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-07-01
Series:Frontiers in Microbiology
Subjects:
DsRed
GFP
fluorescent reporter
biocontrol
root colonization
fungal transformation
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.01653/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2018.01653/full

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