Design of primers and optimization of PCR conditions for the detection of alternatively spliced isoforms of mouse ChAT mRNA

Acetylcholine plays an important role as a neurotransmitter in the central and peripheral nervous systems. Its biosynthesis is catalyzed by choline acetyltransferase (ChAT). In mice, the ChAT gene has three 5′-noncoding (R, N, and M) and 14 coding exons, from which seven mRNA isoforms...

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Bibliographic Details
Main Authors: Desislava Marinova, Stefan Trifonov
Format: Article
Language:English
Published: Medical University - Pleven 2024-06-01
Series:Journal of Biomedical & Clinical Research
Subjects:
Online Access:https://jbcr.arphahub.com/article/121803/download/pdf/