Data on the optimization of an archaea-specific probe-based qPCR assay

Estimation of archaeal numbers by use of fluorescent DNA binding dyes is challenging, because primers targeting the archaeal 16SrRNA genes readily also bind to bacterial 16S rRNA gene sequences, especially when the relative abundance of bacteria is greater than that of archaea. In order to increase...

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Bibliographic Details
Main Authors: Malin Bomberg, Hanna Miettinen
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:Data in Brief
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340920314906