Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assay
The objective of the present study was to estimate applied doses of irradiated anchovy (1.83−4.22 kGy) and bluefish (1.98−5.40 kGy) for shelf-life extension by using image analysis in combination with comet assay for trading irradiated fish, especially for public health concerns. Comet assay was car...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2023-02-01
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| Series: | Food Materials Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/fmr-0023-0032 |
| _version_ | 1797293270149103616 |
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| author | Nurcan Cetinkaya Erhan Ic Yakup Erel Demet Ercin Sumer Ozvatan Nizamettin Yazici |
| author_facet | Nurcan Cetinkaya Erhan Ic Yakup Erel Demet Ercin Sumer Ozvatan Nizamettin Yazici |
| author_sort | Nurcan Cetinkaya |
| collection | DOAJ |
| description | The objective of the present study was to estimate applied doses of irradiated anchovy (1.83−4.22 kGy) and bluefish (1.98−5.40 kGy) for shelf-life extension by using image analysis in combination with comet assay for trading irradiated fish, especially for public health concerns. Comet assay was carried out considering the EN 13784 protocol. Observed comets were evaluated and recorded by the use of image analysis software (BS 200 ProP). Comets with long tails were observed in the irradiated anchovy and bluefish tissues. However, limited numbers of comets in intact cells were determined in the unirradiated samples. The obtained results clearly indicated that head diameter, head DNA, tail DNA, tail length, and tail moment parameters showed very good correlation (R2 = 0.82 and 0.77 for anchovy and bluefish respectively) with the applied doses of irradiation. Using image analysis in combination with comet assay may be a very useful tool for quantification of absorbed doses for local market checks and also in imported irradiated fish for official control at customs. |
| first_indexed | 2024-03-07T20:10:27Z |
| format | Article |
| id | doaj.art-fcb252a0e2084a808f933c83734d9bc5 |
| institution | Directory Open Access Journal |
| issn | 2771-4683 |
| language | English |
| last_indexed | 2024-03-07T20:10:27Z |
| publishDate | 2023-02-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Food Materials Research |
| spelling | doaj.art-fcb252a0e2084a808f933c83734d9bc52024-02-28T01:36:14ZengMaximum Academic PressFood Materials Research2771-46832023-02-01311610.48130/fmr-0023-0032fmr-0023-0032Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assayNurcan Cetinkaya0Erhan Ic1Yakup Erel2Demet Ercin3Sumer Ozvatan4Nizamettin Yazici5Ondokuz Mayıs University, Faculty of Veterinary Medicine, 55200, Samsun, TürkiyeTurkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, 06980, Ankara, TürkiyeTurkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, 06980, Ankara, TürkiyeTurkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, 06980, Ankara, TürkiyeTurkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, 06980, Ankara, TürkiyeTurkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, 06980, Ankara, TürkiyeThe objective of the present study was to estimate applied doses of irradiated anchovy (1.83−4.22 kGy) and bluefish (1.98−5.40 kGy) for shelf-life extension by using image analysis in combination with comet assay for trading irradiated fish, especially for public health concerns. Comet assay was carried out considering the EN 13784 protocol. Observed comets were evaluated and recorded by the use of image analysis software (BS 200 ProP). Comets with long tails were observed in the irradiated anchovy and bluefish tissues. However, limited numbers of comets in intact cells were determined in the unirradiated samples. The obtained results clearly indicated that head diameter, head DNA, tail DNA, tail length, and tail moment parameters showed very good correlation (R2 = 0.82 and 0.77 for anchovy and bluefish respectively) with the applied doses of irradiation. Using image analysis in combination with comet assay may be a very useful tool for quantification of absorbed doses for local market checks and also in imported irradiated fish for official control at customs.https://www.maxapress.com/article/doi/10.48130/fmr-0023-0032anchovybluefishdnacomet assayirradiation. |
| spellingShingle | Nurcan Cetinkaya Erhan Ic Yakup Erel Demet Ercin Sumer Ozvatan Nizamettin Yazici Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assay Food Materials Research anchovy bluefish dna comet assay irradiation. |
| title | Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assay |
| title_full | Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assay |
| title_fullStr | Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assay |
| title_full_unstemmed | Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assay |
| title_short | Estimation of the applied doses in irradiated anchovy and bluefish for shelf-life extension using image analysis in combination with DNA comet assay |
| title_sort | estimation of the applied doses in irradiated anchovy and bluefish for shelf life extension using image analysis in combination with dna comet assay |
| topic | anchovy bluefish dna comet assay irradiation. |
| url | https://www.maxapress.com/article/doi/10.48130/fmr-0023-0032 |
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