Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain
The aim of the study was to identify the isolation rate of thermotolerant campylobacters in a small-scale broiler-meat production farm over a one-year period. The second deliverable of the study was to determine the potential virulence markers. The laboratory investigation was performed on 283 sampl...
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| Format: | Article |
| Language: | English |
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Ss.Cyril and Methodius University, Skopje
2021-10-01
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| Series: | Macedonian Veterinary Review |
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| Online Access: | https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2021_0020 |
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| author | Zagorka Popova Katerina Blagoevska Sandra Mojsova Marija Ratkova Manovska Mirko Prodanov Dean Jankuloski Pavle Sekulovski |
| author_facet | Zagorka Popova Katerina Blagoevska Sandra Mojsova Marija Ratkova Manovska Mirko Prodanov Dean Jankuloski Pavle Sekulovski |
| author_sort | Zagorka Popova |
| collection | DOAJ |
| description | The aim of the study was to identify the isolation rate of thermotolerant campylobacters in a small-scale broiler-meat production farm over a one-year period. The second deliverable of the study was to determine the potential virulence markers. The laboratory investigation was performed on 283 samples (cloacal swabs, caeca, carcass swabs) collected on three sampling points (farm, slaughter line, and cold storage). The isolates obtained with the conventional microbiological method were confirmed with multiplex PCR for identification of campylobacters. The presence of 10 virulence genes was analyzed in the C. jejuni isolates ( flaA, racR, virB11, dnaJ, wlaN, cadF, ciaB, cdtA, cdtB, cdtC). Out of 283 samples, 169 (59.7%) were confirmed as Campylobacter spp., 111 (39.2%) C. jejuni, and 43 (15.2%) C. coli. C. jejuni was the most prevalent in all sampling points. Campylobacter spp. showed a characteristically seasonal prevalence with the highest isolation rate during the warmer period of the year. We detected the cadF and ciaB genes in all C. jejuni isolates. The flaA gene was present in 50% of the examined strains. The cdt genes (cdtA, cdtB, and cdtC) were confirmed in 52.8%, 52.8%, and 47.2% of the C. jejuni strains, respectively. C. jejuni showed 15 profiles of virulence patterns with four predominant profiles. |
| first_indexed | 2024-12-14T23:56:28Z |
| format | Article |
| id | doaj.art-fcb5cf0720a2465ca332623be3362458 |
| institution | Directory Open Access Journal |
| issn | 1409-7621 1857-7415 |
| language | English |
| last_indexed | 2024-12-14T23:56:28Z |
| publishDate | 2021-10-01 |
| publisher | Ss.Cyril and Methodius University, Skopje |
| record_format | Article |
| series | Macedonian Veterinary Review |
| spelling | doaj.art-fcb5cf0720a2465ca332623be33624582022-12-21T22:43:06ZengSs.Cyril and Methodius University, SkopjeMacedonian Veterinary Review1409-76211857-74152021-10-0144214915710.2478/macvetrev-2021-0020Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chainZagorka Popova0Katerina Blagoevska1Sandra Mojsova2Marija Ratkova Manovska3Mirko Prodanov4Dean Jankuloski5Pavle Sekulovski6Faculty of Veterinary Medicine – Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North MacedoniaFaculty of Veterinary Medicine – Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North MacedoniaFaculty of Veterinary Medicine – Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North MacedoniaFaculty of Veterinary Medicine – Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North MacedoniaFaculty of Veterinary Medicine – Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North MacedoniaFaculty of Veterinary Medicine – Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North MacedoniaFaculty of Veterinary Medicine – Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North MacedoniaThe aim of the study was to identify the isolation rate of thermotolerant campylobacters in a small-scale broiler-meat production farm over a one-year period. The second deliverable of the study was to determine the potential virulence markers. The laboratory investigation was performed on 283 samples (cloacal swabs, caeca, carcass swabs) collected on three sampling points (farm, slaughter line, and cold storage). The isolates obtained with the conventional microbiological method were confirmed with multiplex PCR for identification of campylobacters. The presence of 10 virulence genes was analyzed in the C. jejuni isolates ( flaA, racR, virB11, dnaJ, wlaN, cadF, ciaB, cdtA, cdtB, cdtC). Out of 283 samples, 169 (59.7%) were confirmed as Campylobacter spp., 111 (39.2%) C. jejuni, and 43 (15.2%) C. coli. C. jejuni was the most prevalent in all sampling points. Campylobacter spp. showed a characteristically seasonal prevalence with the highest isolation rate during the warmer period of the year. We detected the cadF and ciaB genes in all C. jejuni isolates. The flaA gene was present in 50% of the examined strains. The cdt genes (cdtA, cdtB, and cdtC) were confirmed in 52.8%, 52.8%, and 47.2% of the C. jejuni strains, respectively. C. jejuni showed 15 profiles of virulence patterns with four predominant profiles.https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2021_0020isolation ratecampylobacter spp.virulencecampylobacter jejunibroiler chain |
| spellingShingle | Zagorka Popova Katerina Blagoevska Sandra Mojsova Marija Ratkova Manovska Mirko Prodanov Dean Jankuloski Pavle Sekulovski Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain Macedonian Veterinary Review isolation rate campylobacter spp. virulence campylobacter jejuni broiler chain |
| title | Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain |
| title_full | Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain |
| title_fullStr | Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain |
| title_full_unstemmed | Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain |
| title_short | Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain |
| title_sort | isolation rate of campylobacter spp and detection of virulence genes of campylobacter jejuni across the broiler chain |
| topic | isolation rate campylobacter spp. virulence campylobacter jejuni broiler chain |
| url | https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2021_0020 |
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