Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging

Multiphoton microscopy requires precise increases in excitation power with imaging depth to generate contrast without damaging the sample. Here the authors show how an adaptive illumination function can be learned from the sample’s shape and used for in vivo imaging of whole lymph nodes.

Dettagli Bibliografici
Autori principali: Henry Pinkard, Hratch Baghdassarian, Adriana Mujal, Ed Roberts, Kenneth H. Hu, Daniel Haim Friedman, Ivana Malenica, Taylor Shagam, Adam Fries, Kaitlin Corbin, Matthew F. Krummel, Laura Waller
Natura: Articolo
Lingua:English
Pubblicazione: Nature Portfolio 2021-03-01
Serie:Nature Communications
Accesso online:https://doi.org/10.1038/s41467-021-22246-5