Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量)

Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量)

以谷氨酸棒杆菌Corynebacterium glutamicum ATCC13032基因组为模板，通过PCR扩增，得到大小为1434bp的谷氨酰胺合成酶基因glnA.以大肠杆菌/谷氨酸棒杆菌穿梭质粒pXMJ19为载体，将扩增得到的目的基因片段克隆至C. glutamicum Res167，获得重组菌株C. glutamicum Res167/pXMJ19-glnA.在摇瓶发酵水平上，通过IPTG诱导glnA基因的表达，并采用反相高效液相色谱方法测定了发酵液中的L-谷氨酰胺含量.结果显示，与未经诱导的对照菌相比，诱导后的重组菌发酵液中的L-谷氨酰胺产量提高了1.8倍，最高产量达1.54 g•...

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Bibliographic Details
Main Authors: ZHENGQiang(郑强), RUANHong(阮红), XUZhi-nan(徐志南), YANGWei-jun(杨卫军)
Format: Article
Language:zho
Published: Zhejiang University Press 2008-11-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
谷氨酸棒杆菌
谷氨酰胺合成酶基因
过量表达
l-谷氨酰胺
Online Access:https://doi.org/10.3785/j.issn.1008-9497.2008.06.020

Internet

https://doi.org/10.3785/j.issn.1008-9497.2008.06.020

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