Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量)
以谷氨酸棒杆菌Corynebacterium glutamicum ATCC13032基因组为模板，通过PCR扩增，得到大小为1434bp的谷氨酰胺合成酶基因glnA.以大肠杆菌/谷氨酸棒杆菌穿梭质粒pXMJ19为载体，将扩增得到的目的基因片段克隆至C. glutamicum Res167，获得重组菌株C. glutamicum Res167/pXMJ19-glnA.在摇瓶发酵水平上，通过IPTG诱导glnA基因的表达，并采用反相高效液相色谱方法测定了发酵液中的L-谷氨酰胺含量.结果显示，与未经诱导的对照菌相比，诱导后的重组菌发酵液中的L-谷氨酰胺产量提高了1.8倍，最高产量达1.54 g•...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2008-11-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
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| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2008.06.020 |
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| author | ZHENGQiang(郑强) RUANHong(阮红) XUZhi-nan(徐志南) YANGWei-jun(杨卫军) |
| author_facet | ZHENGQiang(郑强) RUANHong(阮红) XUZhi-nan(徐志南) YANGWei-jun(杨卫军) |
| author_sort | ZHENGQiang(郑强) |
| collection | DOAJ |
| description | 以谷氨酸棒杆菌Corynebacterium glutamicum ATCC13032基因组为模板，通过PCR扩增，得到大小为1434bp的谷氨酰胺合成酶基因glnA.以大肠杆菌/谷氨酸棒杆菌穿梭质粒pXMJ19为载体，将扩增得到的目的基因片段克隆至C. glutamicum Res167，获得重组菌株C. glutamicum Res167/pXMJ19-glnA.在摇瓶发酵水平上，通过IPTG诱导glnA基因的表达，并采用反相高效液相色谱方法测定了发酵液中的L-谷氨酰胺含量.结果显示，与未经诱导的对照菌相比，诱导后的重组菌发酵液中的L-谷氨酰胺产量提高了1.8倍，最高产量达1.54 g• L-1. |
| first_indexed | 2024-04-24T17:00:09Z |
| format | Article |
| id | doaj.art-fcc78b18eebb43af984c6f8d31ea30ed |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:00:09Z |
| publishDate | 2008-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-fcc78b18eebb43af984c6f8d31ea30ed2024-03-29T01:58:26ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972008-11-0135667868310.3785/j.issn.1008-9497.2008.06.020Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量)ZHENGQiang(郑强)0RUANHong(阮红)1XUZhi-nan(徐志南)2YANGWei-jun(杨卫军)3 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江 杭州 310058) 2.College of Medical and Life Science, City College of Zhejiang University, Hangzhou 310015, China( 2.浙江大学城市学院医学与生命科学学院，浙江 杭州 310015) 3.College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China( 3.浙江大学材料与化学工程学院，浙江 杭州 310027) 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江 杭州 310058)以谷氨酸棒杆菌Corynebacterium glutamicum ATCC13032基因组为模板，通过PCR扩增，得到大小为1434bp的谷氨酰胺合成酶基因glnA.以大肠杆菌/谷氨酸棒杆菌穿梭质粒pXMJ19为载体，将扩增得到的目的基因片段克隆至C. glutamicum Res167，获得重组菌株C. glutamicum Res167/pXMJ19-glnA.在摇瓶发酵水平上，通过IPTG诱导glnA基因的表达，并采用反相高效液相色谱方法测定了发酵液中的L-谷氨酰胺含量.结果显示，与未经诱导的对照菌相比，诱导后的重组菌发酵液中的L-谷氨酰胺产量提高了1.8倍，最高产量达1.54 g• L-1.https://doi.org/10.3785/j.issn.1008-9497.2008.06.020谷氨酸棒杆菌谷氨酰胺合成酶基因过量表达l-谷氨酰胺 |
| spellingShingle | ZHENGQiang(郑强) RUANHong(阮红) XUZhi-nan(徐志南) YANGWei-jun(杨卫军) Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量) Zhejiang Daxue xuebao. Lixue ban 谷氨酸棒杆菌 谷氨酰胺合成酶基因 过量表达 l-谷氨酰胺 |
| title | Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量) |
| title_full | Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量) |
| title_fullStr | Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量) |
| title_full_unstemmed | Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量) |
| title_short | Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量) |
| title_sort | significantly improving the productivity of glutamine with over expression of glna in corynebacterium glutamicum 谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中l 谷氨酰胺产量 |
| topic | 谷氨酸棒杆菌 谷氨酰胺合成酶基因 过量表达 l-谷氨酰胺 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2008.06.020 |
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