Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量)
以谷氨酸棒杆菌Corynebacterium glutamicum ATCC13032基因组为模板,通过PCR扩增,得到大小为1434bp的谷氨酰胺合成酶基因glnA.以大肠杆菌/谷氨酸棒杆菌穿梭质粒pXMJ19为载体,将扩增得到的目的基因片段克隆至C. glutamicum Res167,获得重组菌株C. glutamicum Res167/pXMJ19-glnA.在摇瓶发酵水平上,通过IPTG诱导glnA基因的表达,并采用反相高效液相色谱方法测定了发酵液中的L-谷氨酰胺含量.结果显示,与未经诱导的对照菌相比,诱导后的重组菌发酵液中的L-谷氨酰胺产量提高了1.8倍,最高产量达1.54 g•...
Main Authors: | ZHENGQiang(郑强), RUANHong(阮红), XUZhi-nan(徐志南), YANGWei-jun(杨卫军) |
---|---|
Format: | Article |
Language: | zho |
Published: |
Zhejiang University Press
2008-11-01
|
Series: | Zhejiang Daxue xuebao. Lixue ban |
Subjects: | |
Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2008.06.020 |
Similar Items
-
转谷氨酰胺酶途径的酶法糖基化修饰在 蛋白质/多肽改性中的研究进展Progress on transglutaminase-catalyzed enzymatic glycosylation in protein/polypeptide modification
by: 王晓杰 WANG Xiaojie
Published: (2022-10-01) -
转谷氨酰胺酶交联时间对玉米胚芽蛋白 功能性质的影响Effect of transglutaminase cross-linking time on functional properties of corn germ protein
by: 王韵仪,任健,宋春丽,孙天颖,宋俊,曹智 WANG Yunyi, REN Jian, SONG Chunli, SUN Tianying, SONG Jun, CAO Zhi
Published: (2022-08-01) -
中国成人2型糖尿病患者γ ‐谷氨酰转移酶浓度与全因死亡率及病因特异性死亡率的关系
by: Haoyu Guan, et al.
Published: (2023-08-01) -
Synthetic Improvement and Charaterization of N-Substituted-2-Chloroacetamides(N-取代-2-氯乙酰胺合成方法的改进及表征)
by: ZHAOHua-rong(赵华绒), et al.
Published: (2010-07-01) -
Synthesis and characterization of N-substitutedphenyl -2-(4, 6-dimethoxypyrimidin-2-ylthio) benzylamines(N-取代苯基-2-(4,6-二甲氧基-2-嘧啶硫基)苄胺的合成和表征)
by: CHENYi-fei(陈一飞), et al.
Published: (2011-03-01)