Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s Disease
Much evidence suggests that both oxidative stress and apoptosis play a key role in the pathogenesis of Parkinson’s disease (PD). The present study aims to evaluate the protective effect of bergamot juice (BJ) against 6-hydroxydopamine (6-OHDA)- or H<sub>2</sub>O<sub>2</sub>-i...
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MDPI AG
2020-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/4/326 |
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| author | Nadia Ferlazzo Santa Cirmi Alessandro Maugeri Caterina Russo Giovanni Enrico Lombardo Sebastiano Gangemi Gioacchino Calapai Vincenzo Mollace Michele Navarra |
| author_facet | Nadia Ferlazzo Santa Cirmi Alessandro Maugeri Caterina Russo Giovanni Enrico Lombardo Sebastiano Gangemi Gioacchino Calapai Vincenzo Mollace Michele Navarra |
| author_sort | Nadia Ferlazzo |
| collection | DOAJ |
| description | Much evidence suggests that both oxidative stress and apoptosis play a key role in the pathogenesis of Parkinson’s disease (PD). The present study aims to evaluate the protective effect of bergamot juice (BJ) against 6-hydroxydopamine (6-OHDA)- or H<sub>2</sub>O<sub>2</sub>-induced cell death. Treatment of differentiated SH-SY5Y human neuroblastoma cells with 6-OHDA or H<sub>2</sub>O<sub>2</sub> resulted in cell death that was significantly reduced by the pre-treatment with BJ. The protective effects of BJ seem to correlate with the reduction of intracellular reactive oxygen species and nitric oxide generation caused by 6-OHDA or H<sub>2</sub>O<sub>2</sub>. BJ also attenuated mitochondrial dysfunction, caspase-3 activation, imbalance of pro- and anti-apoptotic proteins, MAPKs activation and reduced NF-ĸB nuclear translocation evoked by neurotoxic agents. Additionally, BJ exhibited excellent antioxidant capability in <i>cell-free</i> assays. Collectively, our results suggest that BJ exerts neuroprotective effect through the interplay with specific cell targets and its antioxidant activity, making it worthy of consideration for the management of neurodegenerative diseases. |
| first_indexed | 2024-03-10T20:40:35Z |
| format | Article |
| id | doaj.art-fcc8860cb0734b9585feef3f01dfad34 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T20:40:35Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-fcc8860cb0734b9585feef3f01dfad342023-11-19T20:47:09ZengMDPI AGPharmaceutics1999-49232020-04-0112432610.3390/pharmaceutics12040326Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s DiseaseNadia Ferlazzo0Santa Cirmi1Alessandro Maugeri2Caterina Russo3Giovanni Enrico Lombardo4Sebastiano Gangemi5Gioacchino Calapai6Vincenzo Mollace7Michele Navarra8Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98100 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98100 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98100 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98100 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98100 Messina, ItalyDepartment of Clinical and Experimental Medicine, University of Messina, 98100 Messina, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98100 Messina, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98100 Messina, ItalyMuch evidence suggests that both oxidative stress and apoptosis play a key role in the pathogenesis of Parkinson’s disease (PD). The present study aims to evaluate the protective effect of bergamot juice (BJ) against 6-hydroxydopamine (6-OHDA)- or H<sub>2</sub>O<sub>2</sub>-induced cell death. Treatment of differentiated SH-SY5Y human neuroblastoma cells with 6-OHDA or H<sub>2</sub>O<sub>2</sub> resulted in cell death that was significantly reduced by the pre-treatment with BJ. The protective effects of BJ seem to correlate with the reduction of intracellular reactive oxygen species and nitric oxide generation caused by 6-OHDA or H<sub>2</sub>O<sub>2</sub>. BJ also attenuated mitochondrial dysfunction, caspase-3 activation, imbalance of pro- and anti-apoptotic proteins, MAPKs activation and reduced NF-ĸB nuclear translocation evoked by neurotoxic agents. Additionally, BJ exhibited excellent antioxidant capability in <i>cell-free</i> assays. Collectively, our results suggest that BJ exerts neuroprotective effect through the interplay with specific cell targets and its antioxidant activity, making it worthy of consideration for the management of neurodegenerative diseases.https://www.mdpi.com/1999-4923/12/4/326<i>Citrus bergamia</i>Parkinson’s diseaseSH-SY5Y cellsnatural products6-OHDAbergamot |
| spellingShingle | Nadia Ferlazzo Santa Cirmi Alessandro Maugeri Caterina Russo Giovanni Enrico Lombardo Sebastiano Gangemi Gioacchino Calapai Vincenzo Mollace Michele Navarra Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s Disease Pharmaceutics <i>Citrus bergamia</i> Parkinson’s disease SH-SY5Y cells natural products 6-OHDA bergamot |
| title | Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s Disease |
| title_full | Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s Disease |
| title_fullStr | Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s Disease |
| title_full_unstemmed | Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s Disease |
| title_short | Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an In Vitro Model of Parkinson’s Disease |
| title_sort | neuroprotective effect of bergamot juice in 6 ohda induced sh sy5y cell death an in vitro model of parkinson s disease |
| topic | <i>Citrus bergamia</i> Parkinson’s disease SH-SY5Y cells natural products 6-OHDA bergamot |
| url | https://www.mdpi.com/1999-4923/12/4/326 |
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