Protein trans-splicing as a protein ligation tool to study protein structure and function
Protein trans-splicing (PTS) exerted by split inteins is a protein ligation reaction which enables overcoming the barriers of conventional heterologous protein production. We provide an overview of the current state-of-the-art in split intein engineering, as well as the achievements of PTS technolog...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
De Gruyter
2011-06-01
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Series: | Biomolecular Concepts |
Subjects: | |
Online Access: | https://doi.org/10.1515/bmc.2011.014 |