inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes

inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes

Cloning the genes and operons encoding heterologous functions in bacterial hosts is now almost exclusively carried out using plasmid vectors. This has multiple drawbacks, including the need for constant selection and variation in copy numbers. The chromosomal integration of transgenes has always off...

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Bibliographic Details
Main Authors: Ranti Dev Shukla, Ágnes Zvara, Ákos Avramucz, Alona Yu. Biketova, Akos Nyerges, László G. Puskás, Tamás Fehér
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Microorganisms
Subjects:
chromosomal gene cloning
genome editing
transgene expression
lambda-Red-mediated recombination
insertion sequence
mobile element
Online Access:https://www.mdpi.com/2076-2607/10/2/236

Internet

https://www.mdpi.com/2076-2607/10/2/236

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