OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD
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| Format: | Article |
| Language: | English |
| Published: |
HACCP Consulting
2011-07-01
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| Series: | Potravinarstvo |
| Subjects: | |
| Online Access: | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/154 |
| _version_ | 1819122533990924288 |
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| author | Ondrej Revák Lenka Maršálková Jozef Čapla Ľubomír Belej Jozef Golian Radoslav Židek Pavol Bajzík |
| author_facet | Ondrej Revák Lenka Maršálková Jozef Čapla Ľubomír Belej Jozef Golian Radoslav Židek Pavol Bajzík |
| author_sort | Ondrej Revák |
| collection | DOAJ |
| description | <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Normálna tabuľka"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} --> <!--[endif] --> <p class="ZPNormalnyText"><span style="font-size: medium;"><span class="hps"><span style="font-size: 10.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB">European Union Member States</span></span><span style="font-size:10.0pt; font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB">, together <span class="hps">with</span> <span class="hps">a number of countries</span> <span class="hps">around the world</span>, <span class="hps">places great emphasis on</span> <span class="hps">ensuring the</span> <span class="hps">protection</span> <span class="hps">of consumers</span> <span class="hps">as a</span> <span class="hps">potential</span> <span class="hps">food</span> <span class="hps">allergic</span> <span class="hps">from</span> <span class="hps">food allergies</span>. <span class="hps">to</span> <span class="hps">inform consumers</span> <span class="hps">that</span> <span class="hps">their</span> <span class="hps">product</span> <span class="hps">may</span> <span class="hps">contain</span> <span class="hps">any of</span> <span class="hps">the</span> <span class="hps">risk</span> <span class="hps">of allergenic</span> <span class="hps">ingredients</span>. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR<sup>®</sup> Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (<em style="mso-bidi-font-style:normal">Cyprinus carpio</em>), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. <span class="hps">Results</span> of <span class="hps">using</span> <span class="hps">the optimized</span> <span class="hps">SYBR</span> <span class="hps">®</span> <span class="hps">Green</span> <span class="hps">I</span> <span class="hps">Real</span><span class="atn">-</span>Time <span class="hps">PCR</span> <span class="hps">method</span> <span class="hps">for</span> <span class="hps">species identification</span> <span class="hps">common carp</span> <span class="hpsatn">(</span><em style="mso-bidi-font-style: normal">Cyprinus <span class="hps">carpio</span></em>) <span class="hps">indicate to</span> <span class="hps">its</span> <span class="hps">suitability.</span></span></span></p><div><br /></div><div><span style="font-family: 'Times New Roman', serif; font-size: 13px; "><strong>doi:10.5219/154</strong></span></div><p> </p> |
| first_indexed | 2024-12-22T06:53:59Z |
| format | Article |
| id | doaj.art-fd20c43a02544b929aaae84411489340 |
| institution | Directory Open Access Journal |
| issn | 1338-0230 1337-0960 |
| language | English |
| last_indexed | 2024-12-22T06:53:59Z |
| publishDate | 2011-07-01 |
| publisher | HACCP Consulting |
| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-fd20c43a02544b929aaae844114893402022-12-21T18:35:02ZengHACCP ConsultingPotravinarstvo1338-02301337-09602011-07-01531510.5219/154OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHODOndrej RevákLenka MaršálkováJozef ČaplaĽubomír BelejJozef GolianRadoslav ŽidekPavol Bajzík<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Normálna tabuľka"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} --> <!--[endif] --> <p class="ZPNormalnyText"><span style="font-size: medium;"><span class="hps"><span style="font-size: 10.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB">European Union Member States</span></span><span style="font-size:10.0pt; font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB">, together <span class="hps">with</span> <span class="hps">a number of countries</span> <span class="hps">around the world</span>, <span class="hps">places great emphasis on</span> <span class="hps">ensuring the</span> <span class="hps">protection</span> <span class="hps">of consumers</span> <span class="hps">as a</span> <span class="hps">potential</span> <span class="hps">food</span> <span class="hps">allergic</span> <span class="hps">from</span> <span class="hps">food allergies</span>. <span class="hps">to</span> <span class="hps">inform consumers</span> <span class="hps">that</span> <span class="hps">their</span> <span class="hps">product</span> <span class="hps">may</span> <span class="hps">contain</span> <span class="hps">any of</span> <span class="hps">the</span> <span class="hps">risk</span> <span class="hps">of allergenic</span> <span class="hps">ingredients</span>. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR<sup>®</sup> Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (<em style="mso-bidi-font-style:normal">Cyprinus carpio</em>), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. <span class="hps">Results</span> of <span class="hps">using</span> <span class="hps">the optimized</span> <span class="hps">SYBR</span> <span class="hps">®</span> <span class="hps">Green</span> <span class="hps">I</span> <span class="hps">Real</span><span class="atn">-</span>Time <span class="hps">PCR</span> <span class="hps">method</span> <span class="hps">for</span> <span class="hps">species identification</span> <span class="hps">common carp</span> <span class="hpsatn">(</span><em style="mso-bidi-font-style: normal">Cyprinus <span class="hps">carpio</span></em>) <span class="hps">indicate to</span> <span class="hps">its</span> <span class="hps">suitability.</span></span></span></p><div><br /></div><div><span style="font-family: 'Times New Roman', serif; font-size: 13px; "><strong>doi:10.5219/154</strong></span></div><p> </p>http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/154species identification, SYBR® Green I. Real-Time PCR , Cyprinus carpio |
| spellingShingle | Ondrej Revák Lenka Maršálková Jozef Čapla Ľubomír Belej Jozef Golian Radoslav Židek Pavol Bajzík OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD Potravinarstvo species identification, SYBR® Green I. Real-Time PCR , Cyprinus carpio |
| title | OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD |
| title_full | OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD |
| title_fullStr | OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD |
| title_full_unstemmed | OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD |
| title_short | OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD |
| title_sort | optimalisation of species identification of common carp cyprinus carpio using sybr r green i real time pcr method |
| topic | species identification, SYBR® Green I. Real-Time PCR , Cyprinus carpio |
| url | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/154 |
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