Anchored linear oligonucleotides: the effective tool for the real-time measurement of uracil DNA glycosylase activity

Base excision repair is one of the important DNA repair mechanisms in cells. The fundamental role in this complex process is played by DNA glycosylases. Here, we present a novel approach for the real-time measurement of uracil DNA glycosylase activity, which employs selected oligonucleotides immobil...

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Bibliographic Details
Main Authors: Anna Ligasová, Ivan Rosenberg, Markéta Bocková, Jiří Homola, Karel Koberna
Format: Article
Language:English
Published: The Royal Society 2021-10-01
Series:Open Biology
Subjects:
Online Access:https://royalsocietypublishing.org/doi/10.1098/rsob.210136