Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens
Abstract Polyphenolics have been predicted to effectively develop antimicrobial agents for the food industry as food additives and promote human health. This study aims to synthesize pomegranate peel extract (PPE) with silver nanoparticles (AgNPs) against eight foodborne pathogens. Multispectroscopi...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46355-x |
| _version_ | 1797630280400371712 |
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| author | Salma M. Farouk Samah H. Abu-Hussien Basma T. Abd-Elhalim Reham M. Mohamed Naira M. Arabe Ahmed A. T. Hussain Mostafa E. Mostafa Bahaa Hemdan Salwa M. El-Sayed Ashraf Bakry Naglaa M. Ebeed Mahmoud Salah Hesham Elhariry Ahmed Galal |
| author_facet | Salma M. Farouk Samah H. Abu-Hussien Basma T. Abd-Elhalim Reham M. Mohamed Naira M. Arabe Ahmed A. T. Hussain Mostafa E. Mostafa Bahaa Hemdan Salwa M. El-Sayed Ashraf Bakry Naglaa M. Ebeed Mahmoud Salah Hesham Elhariry Ahmed Galal |
| author_sort | Salma M. Farouk |
| collection | DOAJ |
| description | Abstract Polyphenolics have been predicted to effectively develop antimicrobial agents for the food industry as food additives and promote human health. This study aims to synthesize pomegranate peel extract (PPE) with silver nanoparticles (AgNPs) against eight foodborne pathogens. Multispectroscopic analysis of UV–vis spectroscopy, Zeta potential, Fourier transform infrared (FTIR) and scanning electron microscopy (SEM) analysis were used to characterize the interaction between PPE and AgNPs. Eight foodborne pathogenic strains (six bacterial and two fungal strains) Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 8379, Klebsiella pneumoniae ATCC 00607, Salmonella typhi DSM 17058, Shigella sonnei DSM 5570, Aspergillus flavus ATCC 9643, and Rhizopus oryzae ATCC 96382 were used to test the inhibitory potential of PPW-AgNPs. The reaction colour of PPE-AgNPs from yellow to brown indicated that the nanoparticles were successfully formed. The UV absorption of PPE-AgNPs was detected at 440 nm of 0.9 SPR. SEM image of PPE-AgNPs exhibited spherical shapes with a zeta potential of − 20.1 mV. PPE-AgNPs showed high antimicrobial activity against all tested strains. The highest inhibition activity of PPE-AgNPs was recorded for the B. subtilis strain followed by K. pneumonia, while the highest resistance was noticed for R. oryzae. The components of pomegranate peel were analyzed using gas chromatography–mass spectrometry (GC–MS). The major constituents of pomegranate peel is phenol (51.1%), followed by Isocitronellol (19.41%) and 1-Propanol, 2-(2-hydroxypropyl)- (16.05%). PPE is key in the simple, eco-friendly green synthesis of extracellular stable AgNPs as an alternative source for harmful chemical disinfectants. |
| first_indexed | 2024-03-11T11:06:06Z |
| format | Article |
| id | doaj.art-fd3337500caa4df7ad4056aa8675e00d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T11:06:06Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fd3337500caa4df7ad4056aa8675e00d2023-11-12T12:15:51ZengNature PortfolioScientific Reports2045-23222023-11-0113111410.1038/s41598-023-46355-xBiosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogensSalma M. Farouk0Samah H. Abu-Hussien1Basma T. Abd-Elhalim2Reham M. Mohamed3Naira M. Arabe4Ahmed A. T. Hussain5Mostafa E. Mostafa6Bahaa Hemdan7Salwa M. El-Sayed8Ashraf Bakry9Naglaa M. Ebeed10Mahmoud Salah11Hesham Elhariry12Ahmed Galal13Undergraduate student, Biotechnology Program, Faculty of Agriculture, Ain Shams UniversityDepartment of Agricultural Microbiology, Faculty of Agriculture, Ain Shams UniversityDepartment of Agricultural Microbiology, Faculty of Agriculture, Ain Shams UniversityUndergraduate student, Biotechnology Program, Faculty of Agriculture, Ain Shams UniversityUndergraduate student, Biotechnology Program, Faculty of Agriculture, Ain Shams UniversityUndergraduate student, Biotechnology Program, Faculty of Agriculture, Ain Shams UniversityUndergraduate student, Biotechnology Program, Faculty of Agriculture, Ain Shams UniversityEnvironmental and Climate Change Research Institute, National Research CenterDepartment of Biochemistry, Faculty of Agriculture, Ain Shams UniversityDepartment of Genetics, Faculty of Agriculture, Ain Shams UniversityDepartment of Genetics, Faculty of Agriculture, Ain Shams UniversityDepartment of Environmental Agricultural Science, Faculty of Graduate Studies and Environmental Research, Ain Shams UniversityDepartment of Food Science, Faculty of Agriculture, Ain Shams UniversityDepartment of Poultry Production, Faculty of Agriculture, Ain Shams UniversityAbstract Polyphenolics have been predicted to effectively develop antimicrobial agents for the food industry as food additives and promote human health. This study aims to synthesize pomegranate peel extract (PPE) with silver nanoparticles (AgNPs) against eight foodborne pathogens. Multispectroscopic analysis of UV–vis spectroscopy, Zeta potential, Fourier transform infrared (FTIR) and scanning electron microscopy (SEM) analysis were used to characterize the interaction between PPE and AgNPs. Eight foodborne pathogenic strains (six bacterial and two fungal strains) Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 8379, Klebsiella pneumoniae ATCC 00607, Salmonella typhi DSM 17058, Shigella sonnei DSM 5570, Aspergillus flavus ATCC 9643, and Rhizopus oryzae ATCC 96382 were used to test the inhibitory potential of PPW-AgNPs. The reaction colour of PPE-AgNPs from yellow to brown indicated that the nanoparticles were successfully formed. The UV absorption of PPE-AgNPs was detected at 440 nm of 0.9 SPR. SEM image of PPE-AgNPs exhibited spherical shapes with a zeta potential of − 20.1 mV. PPE-AgNPs showed high antimicrobial activity against all tested strains. The highest inhibition activity of PPE-AgNPs was recorded for the B. subtilis strain followed by K. pneumonia, while the highest resistance was noticed for R. oryzae. The components of pomegranate peel were analyzed using gas chromatography–mass spectrometry (GC–MS). The major constituents of pomegranate peel is phenol (51.1%), followed by Isocitronellol (19.41%) and 1-Propanol, 2-(2-hydroxypropyl)- (16.05%). PPE is key in the simple, eco-friendly green synthesis of extracellular stable AgNPs as an alternative source for harmful chemical disinfectants.https://doi.org/10.1038/s41598-023-46355-x |
| spellingShingle | Salma M. Farouk Samah H. Abu-Hussien Basma T. Abd-Elhalim Reham M. Mohamed Naira M. Arabe Ahmed A. T. Hussain Mostafa E. Mostafa Bahaa Hemdan Salwa M. El-Sayed Ashraf Bakry Naglaa M. Ebeed Mahmoud Salah Hesham Elhariry Ahmed Galal Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens Scientific Reports |
| title | Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens |
| title_full | Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens |
| title_fullStr | Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens |
| title_full_unstemmed | Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens |
| title_short | Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens |
| title_sort | biosynthesis and characterization of silver nanoparticles from punica granatum pomegranate peel waste and its application to inhibit foodborne pathogens |
| url | https://doi.org/10.1038/s41598-023-46355-x |
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