OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGN
An orthogonal designed experiment was used to investigate the effects of sulfite pretreatment on the components separation and saccharification of wheat straw. The process involved sulfite pretreatment of wheat straw under acidic conditions followed by mechanical size reduction using a high consiste...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2011-04-01
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| Series: | BioResources |
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| Online Access: | http://www.ncsu.edu/bioresources/BioRes_06/BioRes_06_2_1414_Yang_WQX_Sulfite_Pretreat_Saccharif_Wheat_Straw_Design_1340.pdf |
| _version_ | 1811256673734492160 |
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| author | Jiayi Yang Gaosheng Wang Lindong Qi Jie Xu |
| author_facet | Jiayi Yang Gaosheng Wang Lindong Qi Jie Xu |
| author_sort | Jiayi Yang |
| collection | DOAJ |
| description | An orthogonal designed experiment was used to investigate the effects of sulfite pretreatment on the components separation and saccharification of wheat straw. The process involved sulfite pretreatment of wheat straw under acidic conditions followed by mechanical size reduction using a high consistency refiner. Reaction temperature, retention time, and charges of sodium bisulfite and sulphuric acid were considered as key factors. The results showed the four factors had impact on saccharification of wheat straw. Raising the temperature, increasing the charge of sodium bisulfite or sulphuric acid, or extending the retention time would improve the dissolution of pentosan, lignin, and saccharification efficiency, while causing further conversion of pentose. The separation of lignin and pentosan from wheat straw was the main cause of improvements in saccharification. With an enzyme loading of 5 FPU cellulase plus 4 CBU β-glucosidase per gram of o.d. substrate, a glucose yield 72.45% was achieved using the substrate pretreated under the conditions of temperature 180 oC, sodium bisulfite charge 3%, sulfuric acid charge 1.48%, and retention time 20 min. |
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| format | Article |
| id | doaj.art-fd389e032e5940fb9b497d772c5dccec |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-04-12T17:44:49Z |
| publishDate | 2011-04-01 |
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| spelling | doaj.art-fd389e032e5940fb9b497d772c5dccec2022-12-22T03:22:42ZengNorth Carolina State UniversityBioResources1930-21262011-04-016214141427OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGNJiayi YangGaosheng WangLindong QiJie XuAn orthogonal designed experiment was used to investigate the effects of sulfite pretreatment on the components separation and saccharification of wheat straw. The process involved sulfite pretreatment of wheat straw under acidic conditions followed by mechanical size reduction using a high consistency refiner. Reaction temperature, retention time, and charges of sodium bisulfite and sulphuric acid were considered as key factors. The results showed the four factors had impact on saccharification of wheat straw. Raising the temperature, increasing the charge of sodium bisulfite or sulphuric acid, or extending the retention time would improve the dissolution of pentosan, lignin, and saccharification efficiency, while causing further conversion of pentose. The separation of lignin and pentosan from wheat straw was the main cause of improvements in saccharification. With an enzyme loading of 5 FPU cellulase plus 4 CBU β-glucosidase per gram of o.d. substrate, a glucose yield 72.45% was achieved using the substrate pretreated under the conditions of temperature 180 oC, sodium bisulfite charge 3%, sulfuric acid charge 1.48%, and retention time 20 min.www.ncsu.edu/bioresources/BioRes_06/BioRes_06_2_1414_Yang_WQX_Sulfite_Pretreat_Saccharif_Wheat_Straw_Design_1340.pdfSulfiteSPORLPretreatmentWheat strawSaccharificationOptimization |
| spellingShingle | Jiayi Yang Gaosheng Wang Lindong Qi Jie Xu OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGN BioResources Sulfite SPORL Pretreatment Wheat straw Saccharification Optimization |
| title | OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGN |
| title_full | OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGN |
| title_fullStr | OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGN |
| title_full_unstemmed | OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGN |
| title_short | OPTIMIZING SULFITE PRRETREATMENT FOR SACCHARIFICATION OF WHEAT STRAW USING ORTHOGONAL DESIGN |
| title_sort | optimizing sulfite prretreatment for saccharification of wheat straw using orthogonal design |
| topic | Sulfite SPORL Pretreatment Wheat straw Saccharification Optimization |
| url | http://www.ncsu.edu/bioresources/BioRes_06/BioRes_06_2_1414_Yang_WQX_Sulfite_Pretreat_Saccharif_Wheat_Straw_Design_1340.pdf |
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