| Summary: | The inhibitory activities of three tea extracts (TEs) and individual phenolic compounds in TEs against porcine pancreatic α-amylase (PPA) were studied by measuring their half inhibitory (IC50) concentrations. The kinetics of inhibition by these extracts and compounds were investigated through Dixon, Cornish-Bowden, and Lineweaver–Burk plots. The results showed that green, oolong and black tea extracts, epigallocatechin gallate, theaflavin-3, 3′-digallate and tannic acid were competitive inhibitors of PPA, whereas epicatechin gallate, theaflavin-3′-gallate and theaflavin were mixed-type inhibitors with both competitive and uncompetitive inhibitory characteristics. Only catechins with a galloyl substituent at the 3-position showed measurable inhibition. The competitive inhibition constants (Kic) were lower for theaflavins than catechins, with the lowest value for theaflavin-3, 3′-digallate. The lower Kic than the uncompetitive inhibition constant for the mixed-type inhibitors suggests that they bind more tightly with free PPA than with the PPA–starch complex. A 3 and/or 3′-galloyl moiety in catechin and theaflavin structures was consistently found to increase inhibition of PPA through enhanced association with the enzyme active site.
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