Quantification and visualization of phosphoinositides by quantum dot-labeled specific binding-domain probes
Phosphoinositides (PI) play important regulatory roles in cell physiology. Localization and quantitation of PIs within the cell is necessary to understand their precise function. Currently, ectopic expression of green fluorescent protein (GFP)-fused PI-binding domains is used to visualize PIs locali...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2012-04-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520405759 |