Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes

Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes

A real-time polymerase chain reaction (PCR) assay with fluorescence resonance energy transfer (FRET) hybridisation probes combined with melting curve analysis was developed to detect Schistosoma japonicum in experimentally infected snails and in faecal samples of infected mice. This procedure is bas...

Full description

Bibliographic Details
Main Authors: Tongjit Thanchomnang, Pewpan Intapan, Pusadee Sri-Aroon, Viraphong Lulitanond, Penchome Janwan, Oranuch Sanpool, Wanchai Maleewong
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2011-11-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
Schistosoma japonicum
real-time fluorescence resonance energy transfer PCR
DNA
snails
mice
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000700008&lng=en&tlng=en

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000700008&lng=en&tlng=en

Similar Items