CRISPR Interference Modules as Low-Burden Logic Inverters in Synthetic Circuits

CRISPR Interference Modules as Low-Burden Logic Inverters in Synthetic Circuits

CRISPR and CRISPRi systems have revolutionized our biological engineering capabilities by enabling the editing and regulation of virtually any gene, via customization of single guide RNA (sgRNA) sequences. CRISPRi modules can work as programmable logic inverters, in which the dCas9-sgRNA complex rep...

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Bibliographic Details
Main Authors: Massimo Bellato, Angelica Frusteri Chiacchiera, Elia Salibi, Michela Casanova, Davide De Marchi, Ignazio Castagliuolo, Maria Gabriella Cusella De Angelis, Paolo Magni, Lorenzo Pasotti
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
CRISPR interference (CRISPRi)
cell load
escherichia coli
synthetic circuits
NOT gate
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2021.743950/full

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https://www.frontiersin.org/articles/10.3389/fbioe.2021.743950/full

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