An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Abstract Background Synthetic biology efforts often require high-throughput screening tools for enzyme engineering campaigns. While innovations in chromatographic and mass spectrometry-based techniques provide relevant structural information associated with enzyme activity, these approaches can requ...

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Bibliographic Details
Main Authors: Brett M. Garabedian, Corey W. Meadows, Florence Mingardon, Joel M. Guenther, Tristan de Rond, Raya Abourjeily, Taek Soon Lee
Format: Article
Language:English
Published: BMC 2020-11-01
Series:Biotechnology for Biofuels
Subjects:
Thin layer chromatography (TLC)
Geranylgeranyl reductase (GGR)
Automation
High-throughput screening (HTS)
Protein engineering
Isoprenoids
Online Access:http://link.springer.com/article/10.1186/s13068-020-01821-w

Internet

http://link.springer.com/article/10.1186/s13068-020-01821-w

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