Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell.
In patients with Parkinson's disease (PD), stem cells can serve as therapeutic agents to restore or regenerate injured nervous system. Here, we differentiated two types of stem cells; mouse embryonic stem cells (mESCs) and protein-based iPS cells (P-iPSCs) generated by non-viral methods, into m...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3899054?pdf=render |
| _version_ | 1819231354299088896 |
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| author | Yoo-Wook Kwon Yeon-Ju Chung Joonoh Kim Ho-Jae Lee Jihwan Park Tae-Young Roh Hyun-Jai Cho Chang-Hwan Yoon Bon-Kwon Koo Hyo-Soo Kim |
| author_facet | Yoo-Wook Kwon Yeon-Ju Chung Joonoh Kim Ho-Jae Lee Jihwan Park Tae-Young Roh Hyun-Jai Cho Chang-Hwan Yoon Bon-Kwon Koo Hyo-Soo Kim |
| author_sort | Yoo-Wook Kwon |
| collection | DOAJ |
| description | In patients with Parkinson's disease (PD), stem cells can serve as therapeutic agents to restore or regenerate injured nervous system. Here, we differentiated two types of stem cells; mouse embryonic stem cells (mESCs) and protein-based iPS cells (P-iPSCs) generated by non-viral methods, into midbrain dopaminergic (mDA) neurons, and then compared the efficiency of DA neuron differentiation from these two cell types. In the undifferentiated stage, P-iPSCs expressed pluripotency markers as ES cells did, indicating that protein-based reprogramming was stable and authentic. While both stem cell types were differentiated to the terminally-matured mDA neurons, P-iPSCs showed higher DA neuron-specific markers' expression than ES cells. To investigate the mechanism of the superior induction capacity of DA neurons observed in P-iPSCs compared to ES cells, we analyzed histone modifications by genome-wide ChIP sequencing analysis and their corresponding microarray results between two cell types. We found that Wnt signaling was up-regulated, while SFRP1, a counter-acting molecule of Wnt, was more suppressed in P-iPSCs than in mESCs. In PD rat model, transplantation of neural precursor cells derived from both cell types showed improved function. The present study demonstrates that P-iPSCs could be a suitable cell source to provide patient-specific therapy for PD without ethical problems or rejection issues. |
| first_indexed | 2024-12-23T11:43:38Z |
| format | Article |
| id | doaj.art-fdd24205a478423f87b2ea096e4c251c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:43:38Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fdd24205a478423f87b2ea096e4c251c2022-12-21T17:48:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8573610.1371/journal.pone.0085736Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell.Yoo-Wook KwonYeon-Ju ChungJoonoh KimHo-Jae LeeJihwan ParkTae-Young RohHyun-Jai ChoChang-Hwan YoonBon-Kwon KooHyo-Soo KimIn patients with Parkinson's disease (PD), stem cells can serve as therapeutic agents to restore or regenerate injured nervous system. Here, we differentiated two types of stem cells; mouse embryonic stem cells (mESCs) and protein-based iPS cells (P-iPSCs) generated by non-viral methods, into midbrain dopaminergic (mDA) neurons, and then compared the efficiency of DA neuron differentiation from these two cell types. In the undifferentiated stage, P-iPSCs expressed pluripotency markers as ES cells did, indicating that protein-based reprogramming was stable and authentic. While both stem cell types were differentiated to the terminally-matured mDA neurons, P-iPSCs showed higher DA neuron-specific markers' expression than ES cells. To investigate the mechanism of the superior induction capacity of DA neurons observed in P-iPSCs compared to ES cells, we analyzed histone modifications by genome-wide ChIP sequencing analysis and their corresponding microarray results between two cell types. We found that Wnt signaling was up-regulated, while SFRP1, a counter-acting molecule of Wnt, was more suppressed in P-iPSCs than in mESCs. In PD rat model, transplantation of neural precursor cells derived from both cell types showed improved function. The present study demonstrates that P-iPSCs could be a suitable cell source to provide patient-specific therapy for PD without ethical problems or rejection issues.http://europepmc.org/articles/PMC3899054?pdf=render |
| spellingShingle | Yoo-Wook Kwon Yeon-Ju Chung Joonoh Kim Ho-Jae Lee Jihwan Park Tae-Young Roh Hyun-Jai Cho Chang-Hwan Yoon Bon-Kwon Koo Hyo-Soo Kim Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell. PLoS ONE |
| title | Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell. |
| title_full | Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell. |
| title_fullStr | Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell. |
| title_full_unstemmed | Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell. |
| title_short | Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell. |
| title_sort | comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein based induced pluripotent stem cell |
| url | http://europepmc.org/articles/PMC3899054?pdf=render |
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