Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model
Abstract Background Primary cytoreductive surgery followed by adjuvant chemotherapy is the standard treatment for advanced epithelial ovarian cancer. The average interval between surgery and chemotherapy initiation is approximately 4-weeks at most centers; however, since surgery may accelerate resid...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-07-01
|
| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-018-0428-6 |
| _version_ | 1797969931600396288 |
|---|---|
| author | Yooyoung Lee Alexandra Kollara Taymaa May Theodore J. Brown |
| author_facet | Yooyoung Lee Alexandra Kollara Taymaa May Theodore J. Brown |
| author_sort | Yooyoung Lee |
| collection | DOAJ |
| description | Abstract Background Primary cytoreductive surgery followed by adjuvant chemotherapy is the standard treatment for advanced epithelial ovarian cancer. The average interval between surgery and chemotherapy initiation is approximately 4-weeks at most centers; however, since surgery may accelerate residual tumor growth, a shorter interval may be more beneficial. Methods The murine ID8 cell model of ovarian cancer was used to examine the efficacy of cisplatin treatment administered perioperatively or 7 days after surgical wounding. Luciferase-expressing cells ID8 cells were injected intraperitoneally (i.p.) into female C57/Bl6 mice. Fourteen days post-injection, animals received an abdominal incision or anesthesia alone and received i.p. cisplatin either on the surgical day or 7 days later, or received no chemotherapy. Additional animals received cisplatin 28 days after wounding for comparison. Results Abdominal tumor mass increased 2.5-fold in wounded vs. unwounded animals as determined by bioluminescent in vivo tumor imaging. Cisplatin administered on the day of wounding decreased tumor burden by 50%, as compared to 90% in unwounded animals. Cisplatin on day 7 or day 28 decreased tumor burden by 80 and 37% respectively. Conclusions Surgical wounding increases ovarian tumor mass and decreases perioperative cisplatin efficacy in this animal model. Administration of cisplatin 1 week after surgery was more effective than cisplatin administered perioperatively or 4 weeks after surgery. |
| first_indexed | 2024-04-11T03:08:59Z |
| format | Article |
| id | doaj.art-fde6a642e0dc4372b08c1e0278f56dae |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:08:59Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-fde6a642e0dc4372b08c1e0278f56dae2023-01-02T12:18:39ZengBMCJournal of Ovarian Research1757-22152018-07-0111111210.1186/s13048-018-0428-6Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse modelYooyoung Lee0Alexandra Kollara1Taymaa May2Theodore J. Brown3Division of Gynecologic Oncology, Princess Margaret Hospital Cancer CentreLunenfeld-Tanenbaum Research Institute at Sinai Health Systems, Mt. Sinai HospitalDivision of Gynecologic Oncology, Princess Margaret Hospital Cancer CentreLunenfeld-Tanenbaum Research Institute at Sinai Health Systems, Mt. Sinai HospitalAbstract Background Primary cytoreductive surgery followed by adjuvant chemotherapy is the standard treatment for advanced epithelial ovarian cancer. The average interval between surgery and chemotherapy initiation is approximately 4-weeks at most centers; however, since surgery may accelerate residual tumor growth, a shorter interval may be more beneficial. Methods The murine ID8 cell model of ovarian cancer was used to examine the efficacy of cisplatin treatment administered perioperatively or 7 days after surgical wounding. Luciferase-expressing cells ID8 cells were injected intraperitoneally (i.p.) into female C57/Bl6 mice. Fourteen days post-injection, animals received an abdominal incision or anesthesia alone and received i.p. cisplatin either on the surgical day or 7 days later, or received no chemotherapy. Additional animals received cisplatin 28 days after wounding for comparison. Results Abdominal tumor mass increased 2.5-fold in wounded vs. unwounded animals as determined by bioluminescent in vivo tumor imaging. Cisplatin administered on the day of wounding decreased tumor burden by 50%, as compared to 90% in unwounded animals. Cisplatin on day 7 or day 28 decreased tumor burden by 80 and 37% respectively. Conclusions Surgical wounding increases ovarian tumor mass and decreases perioperative cisplatin efficacy in this animal model. Administration of cisplatin 1 week after surgery was more effective than cisplatin administered perioperatively or 4 weeks after surgery.http://link.springer.com/article/10.1186/s13048-018-0428-6Ovarian cancerID8 cellsWound healingCisplatinMouseChemoresistance |
| spellingShingle | Yooyoung Lee Alexandra Kollara Taymaa May Theodore J. Brown Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model Journal of Ovarian Research Ovarian cancer ID8 cells Wound healing Cisplatin Mouse Chemoresistance |
| title | Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model |
| title_full | Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model |
| title_fullStr | Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model |
| title_full_unstemmed | Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model |
| title_short | Wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model |
| title_sort | wounding promotes ovarian cancer progression and decreases efficacy of cisplatin in a syngeneic mouse model |
| topic | Ovarian cancer ID8 cells Wound healing Cisplatin Mouse Chemoresistance |
| url | http://link.springer.com/article/10.1186/s13048-018-0428-6 |
| work_keys_str_mv | AT yooyounglee woundingpromotesovariancancerprogressionanddecreasesefficacyofcisplatininasyngeneicmousemodel AT alexandrakollara woundingpromotesovariancancerprogressionanddecreasesefficacyofcisplatininasyngeneicmousemodel AT taymaamay woundingpromotesovariancancerprogressionanddecreasesefficacyofcisplatininasyngeneicmousemodel AT theodorejbrown woundingpromotesovariancancerprogressionanddecreasesefficacyofcisplatininasyngeneicmousemodel |