Regulation of the expression of chaperone gp96 in macrophages and dendritic cells.
The chaperone function of the ER-residing heat shock protein gp96 plays an important role in protein physiology and has additionally important immunological functions due to its peptide-binding capacity. Low amounts of gp96 stimulate immunity; high quantities induce tolerance by mechanisms not fully...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3797789?pdf=render |
| _version_ | 1828202768967401472 |
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| author | Lutz Wolfram Anne Fischbeck Isabelle Frey-Wagner Kacper A Wojtal Silvia Lang Michael Fried Stephan R Vavricka Martin Hausmann Gerhard Rogler |
| author_facet | Lutz Wolfram Anne Fischbeck Isabelle Frey-Wagner Kacper A Wojtal Silvia Lang Michael Fried Stephan R Vavricka Martin Hausmann Gerhard Rogler |
| author_sort | Lutz Wolfram |
| collection | DOAJ |
| description | The chaperone function of the ER-residing heat shock protein gp96 plays an important role in protein physiology and has additionally important immunological functions due to its peptide-binding capacity. Low amounts of gp96 stimulate immunity; high quantities induce tolerance by mechanisms not fully understood. A lack of gp96 protein in intestinal macrophages (IMACs) from Crohn`s disease (CD) patients correlates with loss of tolerance against the host gut flora, leading to chronic inflammation. Since gp96 shows dose-dependent direction of immunological reactions, we studied primary IMACs and developed cell models to understand the regulation of gp96 expression. Induction of gp96-expression was higher in in vitro differentiated dendritic cells (i.v.DCs) than in in vitro differentiated macrophages (i.v.MACs), whereas monocytes (MOs) expressed only low gp96 levels. The highest levels of expression were found in IMACs. Lipopolysaccharide (LPS), muramyl dipeptide (MDP), tumour necrosis factor (TNF), and Interleukin (IL)-4 induced gp96-expression, while IL12, IL-17, IL-23 and interferon (IFN)-γ were not effective indicating that Th1 and Th17 cells are probably not involved in the induction of gp96. Furthermore, gp96 was able to induce its own expression. The ER-stress inducer tunicamycin increased gp96-expression in a concentration- and time-dependent manner. Both ulcerative colitis (UC) and CD patients showed significantly elevated gp96 mRNA levels in intestinal biopsies which correlated positively with the degree of inflammation of the tissue. Since gp96 is highly expressed on the one hand upon stress induction as during inflammation and on the other hand possibly mediating tolerance, these results will help to understand the whether gp96 plays a role in the pathophysiology of inflammatory bowel disease (IBD). |
| first_indexed | 2024-04-12T11:54:22Z |
| format | Article |
| id | doaj.art-fe30a3ce69034bebb006acb5d7981167 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:54:22Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fe30a3ce69034bebb006acb5d79811672022-12-22T03:34:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7635010.1371/journal.pone.0076350Regulation of the expression of chaperone gp96 in macrophages and dendritic cells.Lutz WolframAnne FischbeckIsabelle Frey-WagnerKacper A WojtalSilvia LangMichael FriedStephan R VavrickaMartin HausmannGerhard RoglerThe chaperone function of the ER-residing heat shock protein gp96 plays an important role in protein physiology and has additionally important immunological functions due to its peptide-binding capacity. Low amounts of gp96 stimulate immunity; high quantities induce tolerance by mechanisms not fully understood. A lack of gp96 protein in intestinal macrophages (IMACs) from Crohn`s disease (CD) patients correlates with loss of tolerance against the host gut flora, leading to chronic inflammation. Since gp96 shows dose-dependent direction of immunological reactions, we studied primary IMACs and developed cell models to understand the regulation of gp96 expression. Induction of gp96-expression was higher in in vitro differentiated dendritic cells (i.v.DCs) than in in vitro differentiated macrophages (i.v.MACs), whereas monocytes (MOs) expressed only low gp96 levels. The highest levels of expression were found in IMACs. Lipopolysaccharide (LPS), muramyl dipeptide (MDP), tumour necrosis factor (TNF), and Interleukin (IL)-4 induced gp96-expression, while IL12, IL-17, IL-23 and interferon (IFN)-γ were not effective indicating that Th1 and Th17 cells are probably not involved in the induction of gp96. Furthermore, gp96 was able to induce its own expression. The ER-stress inducer tunicamycin increased gp96-expression in a concentration- and time-dependent manner. Both ulcerative colitis (UC) and CD patients showed significantly elevated gp96 mRNA levels in intestinal biopsies which correlated positively with the degree of inflammation of the tissue. Since gp96 is highly expressed on the one hand upon stress induction as during inflammation and on the other hand possibly mediating tolerance, these results will help to understand the whether gp96 plays a role in the pathophysiology of inflammatory bowel disease (IBD).http://europepmc.org/articles/PMC3797789?pdf=render |
| spellingShingle | Lutz Wolfram Anne Fischbeck Isabelle Frey-Wagner Kacper A Wojtal Silvia Lang Michael Fried Stephan R Vavricka Martin Hausmann Gerhard Rogler Regulation of the expression of chaperone gp96 in macrophages and dendritic cells. PLoS ONE |
| title | Regulation of the expression of chaperone gp96 in macrophages and dendritic cells. |
| title_full | Regulation of the expression of chaperone gp96 in macrophages and dendritic cells. |
| title_fullStr | Regulation of the expression of chaperone gp96 in macrophages and dendritic cells. |
| title_full_unstemmed | Regulation of the expression of chaperone gp96 in macrophages and dendritic cells. |
| title_short | Regulation of the expression of chaperone gp96 in macrophages and dendritic cells. |
| title_sort | regulation of the expression of chaperone gp96 in macrophages and dendritic cells |
| url | http://europepmc.org/articles/PMC3797789?pdf=render |
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