Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

Abstract Background Komagataella phaffii is a commonly used alternative host for manufacturing therapeutic proteins, in part because of its ability to secrete recombinant proteins into the extracellular space. Incorrect processing of secreted proteins by cells can, however, cause non-functional prod...

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Bibliographic Details
Main Authors: Neil C. Dalvie, Christopher A. Naranjo, Sergio A. Rodriguez-Aponte, Ryan S. Johnston, J. Christopher Love
Format: Article
Language:English
Published: BMC 2022-09-01
Series:Microbial Cell Factories
Subjects:
Pichia pastoris
Product quality
Signal peptide
Protein engineering
Aggregation
Online Access:https://doi.org/10.1186/s12934-022-01905-2

Internet

https://doi.org/10.1186/s12934-022-01905-2

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