RAPID SOUTHERN BLOT PROTOCOL FOR THE ANALYSIS OF TARGETED MUTAGENESIS IN THE MOUSE IGHG1 LOCUS
The efficiency of homologous recombination relies on the lenght of the homology arms and the chromatin compactation of the locus. In ES cells the immuoglobulin locu is compacted. Thus, the homologous recombination events occur at a very low frequency. A high number of ES cell colonies have to be ana...
| Auteurs principaux: | , , , |
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| Format: | Article |
| Langue: | English |
| Publié: |
Agroprint Timisoara
2023-11-01
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| Collection: | Scientific Papers Animal Science and Biotechnologies |
| Sujets: | |
| Accès en ligne: | https://spasb.ro/index.php/public_html/article/view/1654 |